Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM83 | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 5 min, slight decrease in activity | Pseudomonas fluorescens | |
PMSF | - |
Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
gel filtration | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from transformed Escherichia coli | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Pseudomonas fluorescens | 1-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Pseudomonas fluorescens | 2-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Pseudomonas fluorescens | 4-nitrophenol + propionate | - |
? | |
benzyl acetate + H2O | - |
Pseudomonas fluorescens | benzyl alcohol + acetate | - |
? | |
benzyl propionate + H2O | - |
Pseudomonas fluorescens | benzyl alcohol + propionate | - |
? | |
beta-phenethyl acetate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
methyl acetate + H2O | - |
Pseudomonas fluorescens | methanol + acetate | - |
? | |
methyl butyrate + H2O | - |
Pseudomonas fluorescens | methanol + butyrate | - |
? | |
methyl caprate + H2O | - |
Pseudomonas fluorescens | methanol + decanoate | - |
? | |
methyl hexanoate + H2O | - |
Pseudomonas fluorescens | methanol + hexanoate | - |
? | |
methyl octanoate + H2O | - |
Pseudomonas fluorescens | methanol + octanoate | - |
? | |
methyl propionate + H2O | no activity | Pseudomonas fluorescens | methanol + propionate | - |
? | |
phenyl acetate + H2O | - |
Pseudomonas fluorescens | phenol + acetate | - |
? | |
triacetin + H2O | - |
Pseudomonas fluorescens | acetic acid + glycerol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Pseudomonas fluorescens |