Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Dudman, N.P.B.; Zerner, B.
    Carboxylesterase from pig and ox liver (1975), Methods Enzymol., 35B, 190-208.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
206000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-15°C, 0.02 M potassium phosphate, pH 7.4, stable for 1.5 years Sus scrofa
4°C, suspension in (NH4)2SO4 solution, stable for 8 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl butyrate + H2O
-
Sus scrofa 2,4-dinitrophenol + butyrate
-
?
2-nitrophenyl butyrate + H2O
-
Sus scrofa 2-nitrophenol + butyrate
-
?
3-nitrophenyl butyrate + H2O
-
Sus scrofa 3-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Sus scrofa 4-nitrophenol + butyrate
-
?
benzyloxycarbonyl-D-Tyr-p-nitrophenyl ester + H2O
-
Sus scrofa benzyloxycarbonyl-D-Tyr + 4-nitrophenol
-
?
benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester + H2O
-
Sus scrofa benzyloxycarbonyl-L-Tyr + 4-nitrophenol
-
?
D-Tyr ethyl ester + H2O
-
Sus scrofa D-Tyr + ethanol
-
?
ethyl butyrate + H2O
-
Sus scrofa ethanol + butyrate
-
?
L-Tyr ethyl ester + H2O
-
Sus scrofa L-Tyr + ethanol
-
?
phenyl acetate + H2O
-
Sus scrofa phenol + acetate
-
?
phenyl acetate + H2O
-
Bos taurus phenol + acetate
-
?
phenyl butyrate + H2O
-
Sus scrofa phenol + butyrate
-
?
phenyl butyrate + H2O
-
Bos taurus phenol + butyrate
-
?
phenyl formate + H2O
-
Sus scrofa phenol + formate
-
?
phenyl pentanoate + H2O
-
Sus scrofa phenol + pentanoate
-
?
phenyl pivalate + H2O
-
Bos taurus phenol + pivalate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Bos taurus
0.9
-
benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
-
Sus scrofa
6.5
-
phenyl trimethyl acetate
-
Bos taurus
24
-
benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
Sus scrofa
71
-
L-Tyr ethyl ester
-
Sus scrofa
71
-
D-Tyr ethyl ester
-
Sus scrofa
233
-
2,4-dinitrophenyl butyrate
-
Sus scrofa
300
-
phenyl acetate
-
Bos taurus
345
-
3-nitrophenyl butyrate
-
Sus scrofa
348
-
o-nitrophenyl butyrate
-
Sus scrofa
367
-
p-nitrophenyl butyrate
-
Sus scrofa
380
-
phenyl acetate
-
Sus scrofa
519
-
phenyl butyrate
-
Sus scrofa
551
-
ethyl butyrate
-
Sus scrofa
660
-
phenyl formate
-
Sus scrofa
860
-
phenyl pentanoate
-
Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.3
-
20°C, 9 h, complete loss of activity Sus scrofa
4.5
-
4°C, 8 h, 30% loss of activity, rapid dissociation to monomeric units Sus scrofa
5
-
7% loss of activity after 7 months Sus scrofa