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Literature summary for 3.1.1.1 extracted from

  • Siddalinga Murthy, K.R.; Veerabhadrappa, P.S.
    Purification, characterization and properties of carboxylesterase from the midgut of the silkworm, Bombyx mori L (1996), Insect Biochem. Mol. Biol., 26, 287-296.
No PubMed abstract available
Search for more literature for 3.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
dichlorvos
-
 Bombyx mori
diethylphosphofluoridate
-
 Bombyx mori
mevinphos
-
 Bombyx mori
p-nitrophenol
-
 Bombyx mori
paraoxon
-
 Bombyx mori
Phosphamidon
-
 Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 1-naphthyl butyrate
-
 Bombyx mori
0.143
-
 1-naphthyl propionate
-
 Bombyx mori
0.37
-
 1-naphthyl acetate
-
 Bombyx mori
0.4
-
 p-nitrophenyl butyrate
-
 Bombyx mori
1.05
-
 4-nitrophenyl propionate
-
 Bombyx mori
2.86
-
 4-nitrophenyl acetate
-
 Bombyx mori
5
-
 2-naphthyl acetate
-
 Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 2 * 72000, SDS-PAGE, gel filtration in presence of 8 M urea Bombyx mori
144500
-
 gel filtration Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
429.5
-
-
 Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Bombyx mori 1-naphthol + acetate
-
 ?
1-naphthyl butyrate + H2O
-
 Bombyx mori 1-naphthol + butyrate
-
 ?
1-naphthyl propionate + H2O
-
 Bombyx mori 1-naphthol + propionate
-
 ?
2-naphthyl acetate + H2O
-
 Bombyx mori 2-naphthol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Bombyx mori 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Bombyx mori 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl propionate + H2O
-
 Bombyx mori 4-nitrophenol + propionate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 72000, SDS-PAGE, gel filtration in presence of 8 M urea Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 20 min, stable Bombyx mori
45
-
 20 min, about 30% loss of activity Bombyx mori
50
-
 20 min, about 50% loss of activity Bombyx mori
55
-
 20 min, about 75 loss of activity Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bombyx mori