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Literature summary for 3.1.1.1 extracted from

  • Saboori, A.M.; Newcombe, D.S.
    Human monocyte carboxylesterase. Purification and kinetics (1990), J. Biol. Chem., 265, 19792-19799.
    View publication on PubMed
Search for more literature for 3.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
ethanol activates Homo sapiens
ethylene glycol little activating effect, except at high concentrations, 1 M Homo sapiens
ethylene glycol monoethyl ether little activating effect, except at high concentrations, 1 M Homo sapiens
methanol activates Homo sapiens
n-butanol 0.1 M, increases activity Homo sapiens

General Stability

General Stability Organism
dialysis using nitrocellulose membranes causes a significant loss of activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Dimethylsulfoxide
-
 Homo sapiens
diphenyl carbonate
-
 Homo sapiens
n-butanol 1 M Homo sapiens
NaF
-
 Homo sapiens
organophosphate compounds
-
 Homo sapiens
paraoxon
-
 Homo sapiens
phenol
-
 Homo sapiens
PMSF
-
 Homo sapiens
triphenyl phosphate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-Nitrophenyl butyrate
-
 Homo sapiens
0.125
-
 4-nitrophenyl pentanoate
-
 Homo sapiens
0.16
-
 1-naphthyl acetate
-
 Homo sapiens
0.33
-
 1-naphthyl butyrate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 3 * 60000, SDS-PAGE Homo sapiens
200000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 at least 4 major isoenzymes in blood monocytes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification contains an N-linked high mannose type carbohydrate chain. Two glucosamine and eight mannose molecules per mol of enzyme Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
monocyte at least 4 major isoenzymes Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 10% glycerol, stable for months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Homo sapiens 1-naphthol + acetate
-
 ?
1-naphthyl butyrate + H2O
-
 Homo sapiens 1-naphthol + butyrate
-
 ?
2-nitrophenyl butyrate + H2O
-
 Homo sapiens 2-nitrophenol + butyrate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Homo sapiens 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl pentanoate + H2O
-
 Homo sapiens 4-nitrophenol + pentanoate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 60000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 hydrolysis of 1-naphthyl butyrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 12 less than 50% of maximal activity at pH less than 6 or more than 12 Homo sapiens