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Literature summary for 2.8.1.15 extracted from

  • Nakagawa, H.; Kuratani, M.; Goto-Ito, S.; Ito, T.; Katsura, K.; Terada, T.; Shirouzu, M.; Sekine, S.; Shigi, N.; Yokoyama, S.
    Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA (2013), Proteins Struct. Funct. Bioinform., 81, 1232-1244.
    View publication on PubMed
Search for more literature for 2.8.1.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus thermophilus
-
 Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.1 A resolution. TtuA forms a homodimer, and each subunit contains a catalytic domain and unique N- and C-terminal zinc fingers. Three conserved cysteine residues are clustered in the putative catalytic site Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
C130S about 10% of wild-type activity Thermus thermophilus
C133S no activity Thermus thermophilus
C222S about 5-10% of wild-type activity Thermus thermophilus
D161A no activity Thermus thermophilus
D59A no activity Thermus thermophilus
E203A about 20% of wild-type activity Thermus thermophilus
H157A about 80% of wild-type activity Thermus thermophilus
K58A about 70% of wild-type activity Thermus thermophilus
N158A about 75% of wild-type activity Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc TtuA has four conserved CXXC motifs and one CXXH motif. In the TtuA structure, the first zinc finger is made up of the first and second CXXC/H motifs, where the zinc atom is coordinated by Cys3, Cys6, Cys22, and His25. The second zinc finger is made up of the fourth and fifth CXXC motifs, where the zinc atom is coordinated by Cys272, Cys275, Cys284, and Cys287 Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35600
-
-
 Pyrococcus horikoshii
70800
-
 analytical ultracentrifugation Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58038
-
-
Pyrococcus horikoshii DSM 12428 O58038
-
-
Thermus thermophilus Q72LF3
-
-
Thermus thermophilus DSM 7039 Q72LF3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Thermus thermophilus AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Pyrococcus horikoshii AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Pyrococcus horikoshii DSM 12428 AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O
-
 Thermus thermophilus DSM 7039 AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35600, calculated Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PH0300
-
 Pyrococcus horikoshii
TtuA
-
 Thermus thermophilus
TtuA
-
 Pyrococcus horikoshii