Literature summary for 2.7.9.3 extracted from

Kamada, S.; Okugochi, T.; Asano, K.; Tobe, R.; Mihara, H.; Nemoto, M.; Inagaki, K.; Tamura, T.
A non-radioactive assay for selenophosphate synthetase activity using recombinant pyruvate pyrophosphate dikinase from Thermus thermophilus HB8 (2016), Biosci. Biotechnol. Biochem., 80, 1970-1972.

Search for more literature for 2.7.9.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene Sephs2, recombinant expression of C-terminally His6-tagged enzyme mutant Sephs2U60C in Escherichia coli Rosetta (DE3) pLysS	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Sec60C	site-directed mutagenesis, the in-frame opal codon for Sec60 is altered to Cys-coding TGT in mutant Sephs2U60C, the substitution of active site Sec with Cys does not significantly perturb the catalytic function of the His6-tagged human Sephs2	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics for the C-terminally His6-tagged enzyme mutant Sephs2-U60C	Homo sapiens
0.026	-	ATP	pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + selenide + H2O	Homo sapiens	-	AMP + selenophosphate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q99611	gene Sephs2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme mutant Sephs2U60C from Escherichia coli Rosetta (DE3) pLysS by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + selenide + H2O	-	Homo sapiens	AMP + selenophosphate + phosphate	-	?
additional information	development of a non-radioactive assay for detection of selenophosphate synthetase activity using recombinant pyruvate diphosphate dikinase, PPDK, from Thermus thermophilus strain HB8 in an enzyme-coupled reaction system, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
Sephs2	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0058	-	ATP	pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)