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Literature summary for 2.7.8.7 extracted from

  • Kim, J.M.; Song, H.Y.; Choi, H.J.; So, K.K.; Kim, D.H.; Chae, K.S.; Han, D.M.; Jahng, K.Y.
    Characterization of NpgA, a 4'-phosphopantetheinyl transferase of Aspergillus nidulans, and evidence of its involvement in fungal growth and formation of conidia and cleistothecia for development (2015), J. Microbiol., 53, 21-31.
    View publication on PubMed
Search for more literature for 2.7.8.7

Cloned(Commentary)

Cloned (Comment) Organism
gene npgA, DNA and amino acid sequence determination and analysis, recombinant overexpression of His6-tagged enzyme in Aspergillus nidulans npgA knockout strain SNT611 using the niiA promoter, and as His6-tagged enzyme in Escherichia coli, functional complementation of Saccharomyces cerevisiae strain BY4741 knockout mutant DPNG Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information generation of a npgA knockout mutant in strain SNT611 Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37700
-
-
 Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Aspergillus nidulans
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Aspergillus nidulans SNT611
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q9UVK7
-
-
Aspergillus nidulans SNT611 Q9UVK7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
cleistothecium
-
 Aspergillus nidulans
-
conidium
-
 Aspergillus nidulans
-
mycelium
-
 Aspergillus nidulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Aspergillus nidulans adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] PPTase cleaves coenzyme A, transfers the P-pant moiety to a conserved residue of inactive substrates, and produces 3'5'-ADP Aspergillus nidulans adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Aspergillus nidulans SNT611 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] PPTase cleaves coenzyme A, transfers the P-pant moiety to a conserved residue of inactive substrates, and produces 3'5'-ADP Aspergillus nidulans SNT611 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?

Subunits

Subunits Comment Organism
? x * 37700, recombinant His6-tagged enzyme, SDS-PAGE Aspergillus nidulans
More enzyme NpgA contains a PPTase superfamily domain, which has a conserved sequence of (V/I)G(V/I)DX(78)(F/W)(S/C/T)XKE(A/S)hhK sequence between aa 187-277, similar to PPTases of other organisms Aspergillus nidulans

Synonyms

Synonyms Comment Organism
4'-phosphopantetheinyl transferase
-
 Aspergillus nidulans
NpgA
-
 Aspergillus nidulans
P-pant transferase
-
 Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Aspergillus nidulans

General Information

General Information Comment Organism
malfunction pleiomorphic phenomena of npgA1 mutant, phenotype, overview Aspergillus nidulans
additional information loss-of-function of LYS5 mutant DNPG, which encodes a PPTase in Saccharomyces cerevisiae strain BY4741, is functionally complemented by NpgA, and Escherichia coli-derived NpgA reveals phosphopantetheinylation activity with the elaboration of 3'5'-ADP Aspergillus nidulans
physiological function the enzyme is essential for growth, and for formation of conidia and cleistothecium in development Aspergillus nidulans