Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
complexed with CoA and acetyl-CoA, and mutant enzymes H110A and D111A, vapor diffusion method. Crystals of the AcpS-CoA-Mg2+ complex are obtained at 18°C, from 0.3 M potassium thiocyanate (KSCN), 0.1 M sodium cacodylate (NaCac) (pH 6.5), and 15% (w/v) PEG 4000. The best diffracting crystal of the AcpS-acetyl-CoA complex is obtained in 0.2 M lithium sulfate, 25% (w/v) PEG 2000 MME, and 0.1 M NaCac (pH 6.5), in the presence of 5 mM acetyl-CoA. The D111A mutant crystallizes in 0.2 M KSCN, 0.1 M NaCac (pH 6.5), 8% (w/v) PEG 20000, and 8% (w/v) PEG 550 MME. The H110A mutant crystal is obtained in 0.3 Ms odium acetate, 0.1 M NaCac (pH 6.5), and 25% (w/v) PEG 2000 MME | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
D111A | active site mutant exhibiting 5% of wild type activity | Streptomyces coelicolor |
E57A | active site mutant without any activity | Streptomyces coelicolor |
H110A | active site mutant exhibiting 28% of wild type activity | Streptomyces coelicolor |
R15A | active site mutant exhibiting 15% of wild type activity | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains Mg2+ | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and Superdex 75 gel filtration | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + apo-[acyl-carrier protein] | - |
Streptomyces coelicolor | CoA + acetyl-[acyl-carrier protein] | - |
? | |
CoA + apo-[acyl-carrier protein] | - |
Streptomyces coelicolor | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Streptomyces coelicolor | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | x-ray crystallography | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
AcpS | - |
Streptomyces coelicolor |
phosphopantetheinyl transferase | - |
Streptomyces coelicolor |
PPTase | - |
Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheine | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
metabolism | AcpS is a doubly promiscuous enzyme capable of activation of acyl-carrier proteins from both fatty acid and polyketide synthesis and catalyzes the transfer of modified CoA substrates | Streptomyces coelicolor |