Literature summary for 2.7.8.7 extracted from

McAllister, K.A.; Peery, R.B.; Meier, T.I.; Fischl, A.S.; Zhao, G.
Biochemical and molecular analyses of the Streptococcus pneumoniae acyl carrier protein synthase, an enzyme essential for fatty acid biosynthesis (2000), J. Biol. Chem., 275, 30864-30872.

Search for more literature for 2.7.8.7

Activating Compound

Activating Compound	Comment	Organism	Structure
apo-acyl-carrier protein	significantly stimulating above 0.01 mM	Streptomyces pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
-	Streptomyces pneumoniae
genes acpS and acpP cloned and overexpressed in Escherichia coli, acpS complements an Escherichia coli mutant	Streptomyces pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
3',5'-ADP	product inhibition	Streptomyces pneumoniae
apo-acyl-carrier protein	slight inhibition at 0.005-0.01 mM	Streptomyces pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0005	-	apo-acyl-carrier protein	pH 7.0, 37°C	Streptomyces pneumoniae
0.0013	-	apo-acyl-carrier protein	pH 7.0, 37°C, trichloroacetic acid precipitation method	Streptomyces pneumoniae
0.0071	-	CoA	pH 7.0, 37°C, trichloroacetic acid precipitation method	Streptomyces pneumoniae
0.0115	-	CoA	pH 7.0, 37°C	Streptomyces pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
10400	-	3 * 10400, homotrimer, SDS-PAGE	Streptomyces pneumoniae
13390	-	AcpS, calculated from predicted amino acid sequence	Streptomyces pneumoniae
13390	-	AcpS, mass spectrometric analysis	Streptomyces pneumoniae
38000	-	purified AcpS, gel filtration	Streptomyces pneumoniae
39000	-	AcpS, sedimentation analysis	Streptomyces pneumoniae
41000	-	AcpS, native homotrimer, predicted from nucleotide sequence	Streptomyces pneumoniae
53000	-	gel filtration	Streptomyces pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA + apo-[acyl-carrier protein]	Streptomyces pneumoniae	transfer of acyl fatty acid intermediates during biosynthesis of fatty acids and lipids in the cell	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r

Organism

Organism	UniProt	Comment	Textmining
Streptomyces pneumoniae	-	-	-
Streptomyces pneumoniae	P0A2W7	apcS gene, open reading frame starting at the second Met codon	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Streptomyces pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetoacetyl-CoA + apo-[acyl-carrier protein]	-	Streptomyces pneumoniae	? + holo-[acyl-carrier protein]	-	r
acetyl-CoA + apo-[acyl-carrier protein]	-	Streptomyces pneumoniae	CoA + acetyl-[acyl-carrier protein]	-	r
CoA + apo-[acyl-carrier protein]	-	Streptomyces pneumoniae	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r
CoA + apo-[acyl-carrier protein]	transfer of acyl fatty acid intermediates during biosynthesis of fatty acids and lipids in the cell	Streptomyces pneumoniae	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r
desulfo-CoA + apo-[acyl-carrier protein]	-	Streptomyces pneumoniae	? + holo-[acyl-carrier protein]	-	r
malonyl-CoA + apo-[acyl-carrier protein]	-	Streptomyces pneumoniae	? + holo-[acyl-carrier protein]	-	r
additional information	dephospho-CoA is no substrate	Streptomyces pneumoniae	?	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 13390, homotrimer, mass spectrometric analysis	Streptomyces pneumoniae
trimer	3 * 10400, homotrimer, SDS-PAGE	Streptomyces pneumoniae

Synonyms

Synonyms	Comment	Organism
AcpS	-	Streptomyces pneumoniae
type II fatty acid synthase system	-	Streptomyces pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	50	-	Streptomyces pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
22	65	stable in this range	Streptomyces pneumoniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Streptomyces pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.006	-	3',5'-ADP	pH 7.0, 37°C	Streptomyces pneumoniae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)