Literature summary for 2.7.8.7 extracted from

Flugel, R.S.; Hwangbo, Y.; Lambalot, R.H.; Cronan, J.E., Jr.; Walsh, C.T.
Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli (2000), J. Biol. Chem., 275, 959-968.

Search for more literature for 2.7.8.7

Cloned(Commentary)

Cloned (Comment)	Organism
gene acpS cloned and expressed in Escherichia coli BL21(DE3)	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
apo-acyl-carrier protein	mutant ACP in which the target serine 36 has been mutated to a threonine residue is an inhibitor as well as an inactive substrate	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	apo-acyl-carrier protein	pH 8.8, 37°C	Escherichia coli
0.05	-	CoA	pH 8.8, 37°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CoA + apo-[acyl-carrier protein]	Escherichia coli	-	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	K-12 derivatives	-
no activity in Escherichia coli	-	denoted acpS1 gene	-
no activity in Escherichia coli MP4	-	denoted acpS1 gene	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CoA + apo-[acyl-carrier protein]	-	Escherichia coli	adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]	-	r
additional information	mutant ACP in which the target serine 36 has been mutated to a threonine residue is an inactive substrate for phosphopantetheinylation	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
AcpS	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.03	-	apo-acyl-carrier protein	pH 8.8, 37°C, 250 mM NaCl	Escherichia coli
1.08	-	apo-acyl-carrier protein	pH 8.8, 37°C	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)