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Literature summary for 2.7.8.7 extracted from

  • Elhussein, S.A.; Miernyk, J.A.; Ohlrogge, J.B.
    Plant holo-(acyl carrier protein) synthase (1988), Biochem. J., 252, 39-45.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.7

General Stability

General Stability Organism
quite heat labile, unstable during chromatographic procedures intended for its purification, can only be partially overcome by Mg2+ and dithiothreitol, addition of ethylene glycol, glycerol, EDTA or proteinase inhibitors failed to stabilize the plant enzyme Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
3',5'-ADP inhibited in vitro by the reaction product Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 apo-acyl-carrier protein pH 8.0, 37°C Spinacia oleracea
0.072
-
 apo-acyl-carrier protein pH 8.0, 37°C Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Spinacia oleracea 5829
-
cytosol
-
 Ricinus communis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires Mg2+ or Mn2+ for activity, Km for Mg2+ 3.0 mM Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + apo-[acyl-carrier protein] Spinacia oleracea
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
CoA + apo-[acyl-carrier protein] Ricinus communis
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
 castor-oil-seed, L. cv. Baker 296
-
Spinacia oleracea
-
 spinach, L. cv. 424
-

Purification (Commentary)

Purification (Comment) Organism
partially Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Ricinus communis
-
leaf
-
 Spinacia oleracea
-
seed
-
 Ricinus communis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + apo-[acyl-carrier protein]
-
 Spinacia oleracea adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
CoA + apo-[acyl-carrier protein]
-
 Ricinus communis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
additional information specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive Spinacia oleracea ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
7 8.8 half maximal activity at pH 7.4 Spinacia oleracea