Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Cricetulus griseus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate | Cricetulus griseus | cardiolipin (phospholipid) biosynthesis, regulation by short chain cell-permeable ceramide (C2-Cer: N-acetylsphingosine) signalling and levels of active GTP-bound RhoA | phosphatidylglycerophosphate + CMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | Q9Z2Z7 | chinese hamster | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO-K1 cell | chinese hamster ovary parental cell line (2.5fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR) and etoposide-/N-acetylsphingosine-resistant cell line E91 (defect in ceramide signalling and RhoGAP activity, non-significant 1.2fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR) | Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
135% increased activity in parental CHO-K1 cell line, stably expressing dominant-negative RhoA(S19N), N-acetylsphingosine-treated, mitochondrial fraction | Cricetulus griseus |
additional information | - |
29% increased activity in CHO-K1 cell line E91, stably expressing dominant-negative RhoA(S19N), N-acetylsphingosine-treated, mitochondrial fraction | Cricetulus griseus |
additional information | - |
64% increased activity in TNFalpha-treated (1 h, 30 nM) parental CHO-K1 cell line, mitochondrial fraction | Cricetulus griseus |
additional information | - |
no change in activity in TNFalpha-treated (1 h, 30 nM) CHO-K1 cell line E91, mitochondrial fraction | Cricetulus griseus |
additional information | - |
slighty decreased constitutive activity in CHO-K1 cell line E91, stably expressing dominant-negative RhoA(S19N), mitochondrial fraction | Cricetulus griseus |
0.000191 | - |
parental CHO-K1 cell line, mitochondrial fraction | Cricetulus griseus |
0.000192 | - |
CHO-K1 cell line E91, mitochondrial fraction | Cricetulus griseus |
0.000197 | - |
CHO-K1 cell line E91, N-acetylsphingosine-treated, no change in activity compared to untreated cells, mitochondrial fraction | Cricetulus griseus |
0.000299 | - |
or 76% increased activity in parental CHO-K1 cell line, N-acetylsphingosine-treated, mitochondrial fraction | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate | cardiolipin (phospholipid) biosynthesis, regulation by short chain cell-permeable ceramide (C2-Cer: N-acetylsphingosine) signalling and levels of active GTP-bound RhoA | Cricetulus griseus | phosphatidylglycerophosphate + CMP | - |
? | |
CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate | in vitro assay on mitochondrial fractions of cells grown in presence or absence of 30-50 microM N-acetylsphingosine | Cricetulus griseus | phosphatidylglycerophosphate + CMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGP synthase | - |
Cricetulus griseus |
phosphatidylglycerolphosphate synthase | - |
Cricetulus griseus |