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Literature summary for 2.7.8.5 extracted from

  • Dowhan, W.
    Phosphatidylglycerophosphate synthase from Escherichia coli (1992), Methods Enzymol., 209, 313-321.
    View publication on PubMed
Search for more literature for 2.7.8.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

General Stability

General Stability Organism
combination of both 4 M urea and 1% SDS at 30°C completely inactivates Escherichia coli
SDS, 1%, 50% loss of activity after 2 h at 30°C Escherichia coli
urea, 8 M, stable in presence of 0.1% Triton X-100 after 2 h at 30°C Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (d)CDP-diacylglycerol 37°C, pH 8.0 Escherichia coli
0.32
-
 sn-glycerol 3-phosphate pH 8.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate ordered sequential bi-bi reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
 37°C, pH 8.0 Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris-HCl, pH 7.0, 10 mM MgCl2, 2 mM DTT, 0.1% Triton X-100, stable for at least 3 years Escherichia coli
4°C, 50 mM Tris-HCl, pH 7.0, 10 mM MgCl2, 2 mM DTT, 0.1% Triton X-100, stable for several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli CMP + phosphatidylglycerophosphate
-
 ?
dCDP-diacylglycerol + sn-glycero-3-phosphate
-
 Escherichia coli dCMP + phosphatidylglycerophosphate
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, stable, partially purified enzyme, Triton X-100 extract Escherichia coli
60
-
 5 min, 50% loss of activity, partially purified enzyme, Triton X-100 extract Escherichia coli
65
-
 5 min, complete loss of activity, partially purified enzyme, Triton X-100 extract Escherichia coli