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Literature summary for 2.7.8.33 extracted from

  • Furlong, S.E.; Valvano, M.A.
    Characterization of the highly conserved VFMGD motif in a bacterial polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase (2012), Protein Sci., 21, 1366-1375.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MV501 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D217A the mutation results in reduced enzymatic activity Escherichia coli
D217E the mutation results in a slightly more active enzyme Escherichia coli
D217K inactive Escherichia coli
D217N the mutation results in a more active enzyme and leads to more than 2fold increase in Vmax without significant change in the Km for the UDP-N-acetyl-D-glucosamine Escherichia coli
D217S the mutation results in a more active enzyme Escherichia coli
F214A the mutation results in severely reduced enzymatic activity Escherichia coli
G216A inactive Escherichia coli
M215A the mutation results in a slightly more active enzyme Escherichia coli
V213A the mutation results in severely reduced enzymatic activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
tunicamycin
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00007
-
 UDP-N-acetyl-D-glucosamine mutant enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37°C Escherichia coli
0.00008
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, in 50 mM of Tris-HCl (pH 8.0), 40 mM of MgCl2, at 37°C Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+ affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate
-
 Escherichia coli UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
 ?

Synonyms

Synonyms Comment Organism
PNPT
-
 Escherichia coli
polyisoprenyl-phosphate N-acetylaminosugar-1-phosphate transferase
-
 Escherichia coli
WecA
-
 Escherichia coli