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Literature summary for 2.7.8.33 extracted from

  • Al-Dabbagh, B.; Mengin-Lecreulx, D.; Bouhss, A.
    Purification and characterization of the bacterial UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase WecA (2008), J. Bacteriol., 190, 7141-7146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
3,6,9,12,15,18-hexaoxatriacontan-1-ol i.e. C12E6, optimal concentration is 90-200 mM Thermotoga maritima
Triton X-100 optimal concentration is 50-120 mM Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pET2130, generating the plasmid pWTM8. The wecA gene product is expressed with an N-terminal polyhistidine tag, expression in Escherichia coli C43(DE3) Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
KCl 125 mM, about 50% of maximal activity Thermotoga maritima
Mg2+ 250 mM, no activity. 10 mM Mg2+ optimally activates the enzyme Thermotoga maritima
NaCl 200 mM, about 50% of maximal activity Thermotoga maritima
tunicamycin 11 nM, 50% inhibition Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
ditrans,polycis-undecaprenyl phosphate pH 8, 65°C Thermotoga maritima
0.62
-
UDP-N-acetyl-D-glucosamine pH 8, 65°C Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Thermotoga maritima 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for a divalent cation (Mg2+), optimal activity at 10 mM, inhibitory at higher conventrations, no activity in absence of Mg2+ Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
SDS-PAGE Thermotoga maritima
33876
-
x * 33876, calculated from sequence Thermotoga maritima
33880
-
calculated from sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate Thermotoga maritima the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1N5
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9X1N5
-
-

Purification (Commentary)

Purification (Comment) Organism
n-dodecyl-beta-D-maltoside is the most efficient detergent for both the extraction and purification steps of the WecA protein Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2895
-
pH 8, 65°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate Thermotoga maritima ?
-
?
additional information no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 ?
-
?
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate a minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate a minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol
-
?
UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol
-
?
UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol
-
?
UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol
-
?
UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol
-
?
UDP-N-acetyl-D-glucosamine + octaprenyl phosphate as active as ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-octaprenol
-
?
UDP-N-acetyl-D-glucosamine + pentadecaprenyl phosphate about 70% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate Thermotoga maritima UMP + N-acetyl-D-glucosaminyldiphospho-pentadecaprenol
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Thermotoga maritima
? x * 33876, calculated from sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase
-
Thermotoga maritima
WecA
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 67 50°C: about 50% of maximal activity, 67°C: about 50% of maximal activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
UDP-N-acetyl-D-glucosamine pH 8, 65°C Thermotoga maritima
1.2
-
ditrans,polycis-undecaprenyl phosphate pH 8, 65°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 50% of maximal activity, pH 9.5: about 70% of maximal activity Thermotoga maritima