Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3,6,9,12,15,18-hexaoxatriacontan-1-ol | i.e. C12E6, optimal concentration is 90-200 mM | Thermotoga maritima | |
Triton X-100 | optimal concentration is 50-120 mM | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
cloned into pET2130, generating the plasmid pWTM8. The wecA gene product is expressed with an N-terminal polyhistidine tag, expression in Escherichia coli C43(DE3) | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 125 mM, about 50% of maximal activity | Thermotoga maritima | |
Mg2+ | 250 mM, no activity. 10 mM Mg2+ optimally activates the enzyme | Thermotoga maritima | |
NaCl | 200 mM, about 50% of maximal activity | Thermotoga maritima | |
tunicamycin | 11 nM, 50% inhibition | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
ditrans,polycis-undecaprenyl phosphate | pH 8, 65°C | Thermotoga maritima | |
0.62 | - |
UDP-N-acetyl-D-glucosamine | pH 8, 65°C | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane protein | Thermotoga maritima | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for a divalent cation (Mg2+), optimal activity at 10 mM, inhibitory at higher conventrations, no activity in absence of Mg2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Thermotoga maritima |
33876 | - |
x * 33876, calculated from sequence | Thermotoga maritima |
33880 | - |
calculated from sequence | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | Thermotoga maritima | the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1N5 | - |
- |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X1N5 | - |
- |
Purification (Comment) | Organism |
---|---|
n-dodecyl-beta-D-maltoside is the most efficient detergent for both the extraction and purification steps of the WecA protein | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2895 | - |
pH 8, 65°C | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate | Thermotoga maritima | ? | - |
? | |
additional information | no activity with farnesyl phosphate and geranylgeranyl phosphate. Heptaprenyl phosphate shows 3% of the activity compared to ditrans,polycis-undecaprenyl phosphate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | a minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | the enzyme catalyzes the first membrane step of the biosynthesis of various polymers of the bacterial cell wall such as the O-antigen and enterobacterial common antigen | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | a minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate | about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + dodecaprenyl phosphate | about 85% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UMP + N-acetyl-D-glucosaminyldiphospho-dodecaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate | about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + heptaprenyl phosphate | about 80% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | UMP + N-acetyl-D-glucosaminyldiphospho-heptaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + octaprenyl phosphate | as active as ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-octaprenol | - |
? | |
UDP-N-acetyl-D-glucosamine + pentadecaprenyl phosphate | about 70% of the activity compared to ditrans,polycis-undecaprenyl phosphate. A minimal length of 35 carbons is required for the lipid substrate | Thermotoga maritima | UMP + N-acetyl-D-glucosaminyldiphospho-pentadecaprenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Thermotoga maritima |
? | x * 33876, calculated from sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase | - |
Thermotoga maritima |
WecA | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 67 | 50°C: about 50% of maximal activity, 67°C: about 50% of maximal activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
UDP-N-acetyl-D-glucosamine | pH 8, 65°C | Thermotoga maritima | |
1.2 | - |
ditrans,polycis-undecaprenyl phosphate | pH 8, 65°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 50% of maximal activity, pH 9.5: about 70% of maximal activity | Thermotoga maritima |