Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EGTA | 0.5 mM, stimulates | Rattus norvegicus | |
ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid | stimulates | Rattus norvegicus | |
Phospholipase C | 23°C, 10 min, slight stimulation | Rattus norvegicus |
General Stability | Organism |
---|---|
freezing and thawing the microsomal preparation 5times destroys activity by 50% | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-butanol | - |
Rattus norvegicus | |
acetone | - |
Rattus norvegicus | |
Ca2+ | - |
Rattus norvegicus | |
CDP | - |
Rattus norvegicus | |
CDPethanolamine | - |
Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
methanol | - |
Rattus norvegicus | |
palmitoyl-CoA | - |
Rattus norvegicus | |
Triton X-100 | WR 1339; X-100 | Rattus norvegicus | |
Trypsin | - |
Rattus norvegicus | |
Tween-20 | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
0.0057 | - |
didodecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0141 | - |
didecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0239 | - |
CDP-choline | cosubstrate 1,2-dioleoyl-sn-glycerol, 23°C, pH 8.5 | Rattus norvegicus | |
0.0314 | - |
dihexadecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0405 | - |
bacterial 1,2-diacyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0501 | - |
dioctadecenoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0509 | - |
1,2-dioleoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.0681 | - |
dihexanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.118 | - |
dimyristoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
0.125 | 0.126 | 1-stearoyl-2-oleoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus | |
Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | - |
Rattus norvegicus | - |
brain | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | fetal | Rattus norvegicus | - |
mucosa | of intestine | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-choline + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylcholine | - |
? | |
CDP-choline + 1,2-dioleoyl-sn-glycerol | - |
Rattus norvegicus | CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine | - |
? | |
CDP-choline + 1-stearoyl-2-oleoyl-sn-glycerol | - |
Rattus norvegicus | CMP + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
CDP-choline + bacterial 1,2-diacyl-sn-glycerol | - |
Rattus norvegicus | CMP + phosphatidylethanolamine | - |
? | |
CDP-choline + didecanoylglycerol | - |
Rattus norvegicus | CMP + didecanoylglycerophosphocholine | - |
? | |
CDP-choline + didodecanoylglycerol | - |
Rattus norvegicus | CMP + didodecanoylglycerophosphocholine | - |
? | |
CDP-choline + dihexanoylglycerol | - |
Rattus norvegicus | CMP + dihexanoylglycerophosphocholine | - |
? | |
CDP-choline + dimyristoyl-sn-glycerol | - |
Rattus norvegicus | CMP + dimyristoyl-sn-glycerophosphocholine | - |
? | |
additional information | specificity overview | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: 1,3-diC18:1-glycerol ether | Rattus norvegicus | ? | - |
? | |
additional information | no substrate: 1,2-diC18:1-glycerol ether | Rattus norvegicus | ? | - |
? | |
additional information | substrates are 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length | Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
19 min, stable, microsomal preparation | Rattus norvegicus |
49 | - |
15 min, 50% loss of activity, microsomal preparation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.3 | - |
Rattus norvegicus |