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Literature summary for 2.7.8.15 extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Purification and properties of UDP-GlcNAc:dolichyl-phosphate GlcNAc-1-phosphate transferase. Activation and inhibition of the enzyme (1985), J. Biol. Chem., 260, 16303-16309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dolichyl-phosphoryl-mannose stimulates Sus scrofa
GDP-mannose stimulates by protecting the substrate UDP-GlcNAc from degradation Sus scrofa
phosphatidylglycerol solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
phosphatidylinositol solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
phosphatidylserine solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
Phospholipid solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
NEM protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate and Mn2+ are added before addition of the sulfhydryl reagent Sus scrofa
PCMB protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate anf Mn2+ are added before addition of the sulfhydryl reagent Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
UDP-GlcNAc pH 7.5, 37°C Sus scrofa
0.001
-
dolichyl phosphate pH 7.5, 37°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ less effective than Mn2+ or Mg2+ Sus scrofa
Mg2+ either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity Sus scrofa
Mn2+ either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
aorta
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
0-4°C, 20% glycerol, 0.02 mg phosphatidylglycerol/mg of protein, less than 20% loss of activity after 6 days. In absence of both glycerol and phosphatidylglycerol the enzyme is quite unstable and loses most of its activity within 24 h Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
Sus scrofa UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
Sus scrofa