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Literature summary for 2.7.8.13 extracted from

  • Ishizaki, Y.; Hayashi, C.; Inoue, K.; Igarashi, M.; Takahashi, Y.; Pujari, V.; Crick, D.C.; Brennan, P.J.; Nomoto, A.
    Inhibition of the first step in synthesis of the mycobacterial cell wall core, catalyzed by the GlcNAc-1-phosphate transferase WecA, by the novel caprazamycin derivative CPZEN-45 (2013), J. Biol. Chem., 288, 30309-30319.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
caprazamycin B
-
Bacillus subtilis
CPZEN-45 CPZEN-45 strongly inhibits incorporation of radiolabeled glycerol into growing cultures and shows antibacterial activity against caprazamycin-resistant strains, including a strain overexpressing translocase-I MraY, involved in the biosynthesis of peptidoglycan, the target of the caprazamycins. Very poor inhibition by vancomycin Bacillus subtilis
tunicamycin
-
Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate Bacillus subtilis
-
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
?
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate Bacillus subtilis 168
-
UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis Q03521 gene mraY
-
Bacillus subtilis 168 Q03521 gene mraY
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate
-
Bacillus subtilis UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
?
UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate
-
Bacillus subtilis 168 UMP + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
?

Synonyms

Synonyms Comment Organism
MraY
-
Bacillus subtilis
translocase-I
-
Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
pH and temperature not specified in the publication Bacillus subtilis caprazamycin B
0.0004
-
pH and temperature not specified in the publication Bacillus subtilis CPZEN-45

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of peptidoglycan Bacillus subtilis