Cloned (Comment) | Organism |
---|---|
in medium-copy plasmid pBAD30 for complementation studies in Escherichia coli K-12 mutant strain RY3321 lacking endogenous MraY | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D231N | catalytically inactive, overexpression does not rescue bacteria from protein E-induced lysis | Bacillus subtilis |
D267N | enzymatically inactive, overexpression rescues bacteria from protein E-induced lysis | Escherichia coli |
deltaL172 | single-codon deletion in putative transmembrane domain TMD5, mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy | Escherichia coli |
F288L | missense mutation in putative transmembrane domain TMD9, mediates PhiX174-resistancy only in catalytically active form | Escherichia coli |
G168S | mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type | Escherichia coli |
P170L | mediates PhiX174-resistancy only in catalytically active form and at high culture densitiy, interacts with Epos gene product more strongly than with protein E | Escherichia coli |
V291M | mediates PhiX174-resistancy only at high culture densitiy, similar to wild-type | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
protein E | lysis protein of bacteriophage PhiX174, (strong) binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis | Bacillus subtilis | |
protein E | lysis protein of bacteriophage PhiX174, binding to MraY leads to cell lysis, overexpression of MraY wild-type rescues bacteria from protein E-induced lysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-MurNAc-pentapeptide + undecaprenyl phosphate | Escherichia coli | Lipid I precursor synthesis, murein biosynthesis | UMP + MurNAc-pentapeptide-diphosphoundecaprenol | - |
? | |
UDP-MurNAc-pentapeptide + undecaprenyl phosphate | Bacillus subtilis | Lipid I precursor synthesis, murein biosynthesis | UMP + MurNAc-pentapeptide-diphosphoundecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q03521 | - |
- |
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-MurNAc-pentapeptide + undecaprenyl phosphate | Lipid I precursor synthesis, murein biosynthesis | Escherichia coli | UMP + MurNAc-pentapeptide-diphosphoundecaprenol | - |
? | |
UDP-MurNAc-pentapeptide + undecaprenyl phosphate | Lipid I precursor synthesis, murein biosynthesis | Bacillus subtilis | UMP + MurNAc-pentapeptide-diphosphoundecaprenol | - |
? | |
UDP-MurNAc-pentapeptide + undecaprenyl phosphate | efficient complementation of enzymatic activity in bacterial strain lacking endogenous MraY | Bacillus subtilis | UMP + MurNAc-pentapeptide-diphosphoundecaprenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MraY | - |
Escherichia coli |
MraY | - |
Bacillus subtilis |
phospho-MurNAc-pentapeptide translocase | - |
Escherichia coli |
phospho-MurNAc-pentapeptide translocase | - |
Bacillus subtilis |
translocase I | - |
Escherichia coli |
translocase I | - |
Bacillus subtilis |