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Literature summary for 2.7.8.13 extracted from

  • Bouhss, A.; Crouvoisier, M.; Blanot, D.; Mengin-Lecreulx, D.
    Purification and characterization of the bacterial MraY translocase catalyzing the first membrane step of peptidoglycan biosynthesis (2004), J. Biol. Chem., 279, 29974-29980.
    View publication on PubMed
Search for more literature for 2.7.8.13

Activating Compound

Activating Compound Comment Organism Structure
additional information N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Staphylococcus aureus
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Bacillus subtilis
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Escherichia coli
His-tagged enzyme, high expression with pET28b expression vector in Escherichia coli BL21(DE3) or C43(DE3), low expression with expression vector pTrc99A in Escherichia coli DH5alpha Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
additional information N-lauroyl sarcosine is the best detergent for translocase activity, among different detergents tested CHAPS is the worst for translocase activity Bacillus subtilis
tunicamycin IC50: 0.012 mM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 undecaprenyl phosphate
-
 Bacillus subtilis
1
-
 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
 Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Bacillus subtilis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for divalent cations, optimal activity with Mg2+ at concentrations 40-75 mM Bacillus subtilis
Mn2+ enzyme activity in presence of Mn2+ is 1% of the activity detected in presence of Mg2+ Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Bacillus subtilis catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Escherichia coli catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Thermotoga maritima catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Staphylococcus aureus catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 recombinant
-
Escherichia coli
-
 recombinant
-
Staphylococcus aureus
-
 recombinant
-
Thermotoga maritima
-
 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Bacillus subtilis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Escherichia coli UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Thermotoga maritima UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Bacillus subtilis UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Escherichia coli UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Thermotoga maritima UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate
-
 Staphylococcus aureus UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r
UDPMur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first membrane step of bacterial cell wall peptidyglycan synthesis Staphylococcus aureus UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
 r

Synonyms

Synonyms Comment Organism
MraY translocase
-
 Staphylococcus aureus
MraY translocase
-
 Bacillus subtilis
MraY translocase
-
 Escherichia coli
MraY translocase
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.33
-
 undecaprenyl phosphate
-
 Bacillus subtilis
5.33
-
 UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)
-
 Bacillus subtilis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
 IC50: 0.012 mM Bacillus subtilis tunicamycin