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Literature summary for 2.7.8.13 extracted from

  • Lloyd, A.J.; Brandish, P.E.; Gilbey, A.M.; Bugg, T.D.H.
    Phospho-N-acetyl-muramyl-pentapeptide translocase from Escherichia coli: catalytic role of conserved aspartic acid residues (2004), J. Bacteriol., 186, 1747-1757.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant enzymes D115N, D116N and D267N as C-terminal hexahistidine fusion proteins Escherichia coli

Protein Variants

Protein Variants Comment Organism
D115N mutatio eliminates translocase 1 activity Escherichia coli
D116N mutatio eliminates translocase 1 activity Escherichia coli
D267N mutatio eliminates translocase 1 activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate Escherichia coli catalyzes the first of a sequence of lipid-linked steps that ultimately assemble the peptidoglycan layer of the bacterial cell wall UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate catalyzes the first of a sequence of lipid-linked steps that ultimately assemble the peptidoglycan layer of the bacterial cell wall Escherichia coli UMP + Mur2Ac(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
-
?

Synonyms

Synonyms Comment Organism
phospho-N-acetyl-muramyl-pentapeptide translocase
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Escherichia coli
translocase 1
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Escherichia coli