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Literature summary for 2.7.8.13 extracted from
- Phospho-N-acetylmuramoyl-pentapeptide-transferase of Escherichia coli K12. Properties of the membrane-bound and the extracted and partially purified enzyme (1978), Biochim. Biophys. Acta, 527, 414-424.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Moenomycin | activates partially purified enzyme, maximal activation at 1 mg moenomycin/mg protein | Escherichia coli |  |
| Phospholipid | phospholipide sensitive to phospholipase necessary for enzymatic activity | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0056 | - |
UDP-NAc-muramoyl-pentapeptide | pH 7.8, 37°C, crude envelopes | Escherichia coli | |
| 0.037 | - |
UDP-NAc-muramoyl-pentapeptide | pH 7.8, 37°C, solubilized enzyme | Escherichia coli | |
| 0.067 | - |
5'-UMP | pH 7.8, 37°C, solubilized enzyme | Escherichia coli | |
| 0.087 | - |
5'-UMP | pH 7.8, 37°C, crude envelopes | Escherichia coli | |
| 0.087 | - |
UDP-MurNAc-pentapeptide | pH 7.8, 37°C, at 0.016 mM C55-isoprenylphosphate | Escherichia coli | |
| 0.27 | - |
UDP-MurNAc-pentapeptide | pH 7.8, 37°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell envelope | - |
Escherichia coli | 30313 | - |
| cytoplasm | - |
Escherichia coli | 5737 | - |
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement, maximun activity at 30-40 mM | Escherichia coli | |
| Mn2+ | can substitute for Mg2+ | Escherichia coli | |
| additional information | Cu2+, Ca2+ and Zn2+ are without effect | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-NAc-muramoyl-pentapeptide + acceptor | Escherichia coli | - |
UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
K12 ATCC 23811 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-MurNAc-pentapeptide + C55-isoprenyl phosphate | - |
Escherichia coli | C55-isoprenyl-diphosphate-MurNAc-pentapeptide | - |
r | |
| UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP | - |
Escherichia coli | UMP + [2H]UDP-NAc-muramoyl-pentapeptide | - |
r | |
| UDP-NAc-muramoyl-pentapeptide + acceptor | - |
Escherichia coli | UMP + acceptor-phospho-NAc-muramoyl-pentapeptide | - |
r | |
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