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Literature summary for 2.7.8.1 extracted from
- Phospholipid synthesis in isolated fat cells. Studies of microsomal diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase activities (1977), J. Biol. Chem., 252, 3050-3056.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 1 mg/ml, 2fold increase of activity | Rattus norvegicus | |
| EGTA | stimulates | Rattus norvegicus |  |
| pospholipase C | 10 min, 23°C, 26% stimulation | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| trypsin, 70%, 0.9 min, activity remains stable | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | - |
Rattus norvegicus | |
| Ca2+ | CaCl2 | Rattus norvegicus | |
| CDP | - |
Rattus norvegicus | |
| CDP-choline | - |
Rattus norvegicus | |
| CMP | - |
Rattus norvegicus | |
| CTP | - |
Rattus norvegicus | |
| dioxane | - |
Rattus norvegicus | |
| dithiothreitol | - |
Rattus norvegicus | |
| methanol | - |
Rattus norvegicus | |
| palmitoyl-CoA | - |
Rattus norvegicus | |
| Triton WR 1339 | - |
Rattus norvegicus | |
| Tween 20 | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0118 | - |
1,2-dioleoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0183 | - |
CDP-ethanolamine | cosubstrate 1,2-dioleoyl-sn-glycerol | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mn2+ or Mg2+ required | Rattus norvegicus | |
| Mg2+ | completely dependent on MgCl2 | Rattus norvegicus | |
| Mn2+ | Mn2+ or Mg2+ required | Rattus norvegicus | |
| Mn2+ | much more effective than Mg2+ | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| mucosa | of intestine | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-ethanolamine + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylethanolamine | - |
? | |
| CDP-ethanolamine + 1,2-dioleoylglycerol | highest activity | Rattus norvegicus | CMP + dioleoylphosphatidylethanolamine | - |
? | |
| CDP-ethanolamine + 1-stearoyl-2-oleoylglycerol | - |
Rattus norvegicus | CMP + 1-stearoyl-2-oleoylphosphatidylethanolamine | - |
? | |
| additional information | 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length are no substrates | Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable for 19 min, heating of microsomes | Rattus norvegicus |
| 49 | - |
4 min, more than 50% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.3 | - |
Rattus norvegicus |
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