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Literature summary for 2.7.7.B3 extracted from

  • Makarchikov, A.F.; Brans, A.; Bettendorff, L.
    Thiamine diphosphate adenylyl transferase from E. coli: functional characterization of the enzyme synthesizing adenosine thiamine triphosphate (2007), BMC Biochem., 8, 17.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B3

Activating Compound

Activating Compound Comment Organism Structure
heat-stable soluble activator requirement for a heat-stable soluble activator present in bacterial extracts Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
bis-Tris-propane
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information varying the ADP concentration does not yield Michealis-Menten kinetics Escherichia coli
4.2
-
 thiamine diphosphate pH 6.5, 37°C, cosubstrate: ADP Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM, requirement for divalent metal ions, Mn2+ is most efficient Escherichia coli
Mn2+ 5 mM, requirement for divalent metal ions, Mg2+ is 70% less effective than Mg2+ Escherichia coli
additional information 5 mM Ca2+ has no effect on activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiamine diphosphate + ADP Escherichia coli biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress adenosine thiamine triphosphate + phosphate
-
 ?
thiamine diphosphate + ATP Escherichia coli biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress adenosine thiamine triphosphate + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00008
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine diphosphate + ADP biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress Escherichia coli adenosine thiamine triphosphate + phosphate
-
 ?
thiamine diphosphate + ADP the enzyme does not accept a thiamine phopshate other than thiamine diphosphate as substrate Escherichia coli adenosine thiamine triphosphate + phosphate
-
 ?
thiamine diphosphate + ATP biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress Escherichia coli adenosine thiamine triphosphate + diphosphate
-
 ?
thiamine diphosphate + ATP the enzyme does not accept a thiamine phopshate other than thiamine diphosphate as substrate Escherichia coli adenosine thiamine triphosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ThDP adenylyl transferase
-
 Escherichia coli
thiamine diphosphate adenylyl transferase
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Escherichia coli