Cloned (Comment) | Organism |
---|---|
cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3) | Carassius auratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
- |
Carassius auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carassius auratus | H9AER4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble His6-tagged enzyme from Escherichia coli strain Rossetta 1 (DE3) by nickel affinity chromatography, identification of the recombinant enzyme by MALDI-TOF-TOF tandem MS analysis | Carassius auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | for examination of the transposition activity of recombinant Tgf2 TPase, microinjection is performed in blunt snout bream embryos at 1-2 cell stage. When donor plasmid pTgf2-EF1alpha-EGFP is co-injected with recombinant Tgf2 TPase protein, 74% of the embryos show almost ubiquitous and uniform expression of EGFP, while 31% control embryos by injected donor plasmid show only mosaic expression of EGFP. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition | Carassius auratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, about, recombinant His6-tagged enzyme, SDS-PAGE | Carassius auratus |
Synonyms | Comment | Organism |
---|---|---|
Tgf2 TPase | - |
Carassius auratus |
Tgf2 transposase | - |
Carassius auratus |
General Information | Comment | Organism |
---|---|---|
evolution | the goldfish Tgf2 transposon belongs to the Hobo/Activator/Tam3 (hAT) family. hAT elements are an ancient family of transposons and are abundant throughout a variety of species | Carassius auratus |