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Literature summary for 2.7.7.B22 extracted from

  • Shen, X.D.; Hou, F.; Chen, J.; Jiang, X.Y.; Zou, S.M.
    Identification of nuclear localization signal within goldfish Tgf2 transposase (2016), Gene, 593, 21-27.
    View publication on PubMed
Search for more literature for 2.7.7.B22

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes from plasmid pEGFP-C1, which contains EGFP driven froma CMV promoter, in HEK-293 cells Carassius auratus

Protein Variants

Protein Variants Comment Organism
additional information construction of C-terminal deletion mutants, i.e. pEGFP-C1-Tgf2TPDELTA31C containing a 31-aa C-terminal deletion of Tgf2 transposase that included the predicted 15-amino acid NLS, pEGFP-C1-Tgf2TPDELTA120N containing a 120-aa N-terminal deletion of Tgf2 transposase, and pEGFP-C1-Tgf2TPDELTA16C containing a 16-aa C-terminal deletion of Tgf2 transposase. Loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus Carassius auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus Carassius auratus
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nucleus 15 aa residues (LLFSPKRARLDTNNF) located from 656 to 670 aa within the C-terminus of Tgf2 transposase can function as a nuclear localization signal (NLS) to assist the transfer of the transposase into the nucleus where it mediates DNA transposition Carassius auratus 5634
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Organism

Organism UniProt Comment Textmining
Carassius auratus H9AER4
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Subunits

Subunits Comment Organism
More amino acid sequence analysis based on Phyre2 prediction suggests that Tgf2 transposase contains an N-terminal zinc finger BED domain (65-120 aa), a helix-turn-helix (HTH) binding structure (163-201 aa) and an RNase-H domain (211-683 aa) with an insertion domain (362-493 aa). The cNLS mapper predicts the presence of a monopartite NLS (656-670 aa) of 15 amino acids (LLFSPKRARLDTNNF) within the RNase-H domain at the C-terminus of Tgf2 transposase. This predicted NLS is located downstream of the DDE residues (D228, D295 and E648). Construction of a 3D model of the NLS and DDE signature of Tgf2 transposase, molecular architecture of full-length Tgf2 transposase overview Carassius auratus

Synonyms

Synonyms Comment Organism
Tgf2 transposase
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 Carassius auratus

General Information

General Information Comment Organism
evolution the goldfish Tgf2 transposon belongs to the Hobo/Activator/Tam3 (hAT) family. hAT elements are an ancient family of transposons and are abundant throughout a variety of species. The NLS of Tgf2 transposase is identical to that of the Tol2 transposase, indicating the evolutionary importance of these sequences for NLS function Carassius auratus
malfunction loss of the nuclear localization signal (NLS) domain results in expression in the cytoplasm but not in the nucleus Carassius auratus
physiological function Tgf2 transposase can mediate efficient gene transfer in teleost fish Carassius auratus