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Literature summary for 2.7.7.B17 extracted from

  • Joshua, C.; Perez, L.; Keasling, J.
    Functional characterization of the origin of replication of pRN1 from Sulfolobus islandicus REN1H1 (2013), PLoS ONE, 8, e84664.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B17

Cloned(Commentary)

Cloned (Comment) Organism
cloning of plasmid pRN1, DNA and amino acid sequence determination and analysis, genetic structure and stem-loop structures within the 232-bp pRN1 putative origin of replication, overview. Quantitative RT-PCR expression analysis, mapping the putative origin of replication of pRN1 Sulfolobus islandicus

Protein Variants

Protein Variants Comment Organism
additional information in-frame deletions of orf 56 and orf904 from pRSP1 generating pRSP10 and pRSP9, respectively. Each of the genes is placed under the control of the putative promoter elements of the cotranscribed genes located at the 5'-end of orf56, in-trans complementation of orf56 and orf904 deletion mutants of pRSP1. Construction of pRN1-based shuttle vectors, comprising orf56 and orf904 as minmal sequence, methylation and transformation, overview Sulfolobus islandicus

Organism

Organism UniProt Comment Textmining
Sulfolobus islandicus Q9C4X5 ORF56; REN1H1, plasmid pRN1, orf 904
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Sulfolobus islandicus E4-39 Q9C4X5 ORF56; REN1H1, plasmid pRN1, orf 904
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Synonyms

Synonyms Comment Organism
ORF904
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 Sulfolobus islandicus
primase
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 Sulfolobus islandicus

General Information

General Information Comment Organism
malfunction plasmids lacking orf904 and orf56 but harboring the putative origin are transformable when orf904 and orf56 are provided in-trans, a 75-bp region 5' of the orf904 start codon is essential for the complementation Sulfolobus islandicus
additional information the loop of the 100-bp structure of the 232-bp region of plasmid pRN1 contains a GTG tri-nucleotide motif, a feature that is important for the primase activity of Orf904. This putative origin and the associated orf56 and orf904 are identified as the minimal replicon of pRN1 Sulfolobus islandicus