Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.B16 extracted from

  • Hu, J.; Guo, L.; Wu, K.; Liu, B.; Lang, S.; Huang, L.
    Template-dependent polymerization across discontinuous templates by the heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus (2012), Nucleic Acids Res., 40, 3470-3483.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B16

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dNTP + n dNTP Saccharolobus solfataricus
-
 dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP Saccharolobus solfataricus P2
-
 dN(pdN)n + n diphosphate
-
 ?
NTP + n NTP Saccharolobus solfataricus
-
 N(pN)n + n diphosphate
-
 ?
NTP + n NTP Saccharolobus solfataricus P2
-
 N(pN)n + n diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UWW1 and Q97Z83 Q9UWW1: small subunit, Q97Z83: large subunit
-
Saccharolobus solfataricus P2 Q9UWW1 and Q97Z83 Q9UWW1: small subunit, Q97Z83: large subunit
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + n ATP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus A(pA)n + n diphosphate
-
 ?
ATP + n ATP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 A(pA)n + n diphosphate
-
 ?
dNTP + n dNTP
-
 Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP
-
 Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
dNTP + n dNTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 dN(pdN)n + n diphosphate
-
 ?
GTP + n GTP the enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus G(pG)n + n diphosphate
-
 ?
NTP + n NTP
-
 Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
NTP + n NTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus N(pN)n + n diphosphate
-
 ?
NTP + n NTP
-
 Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?
NTP + n NTP the enzyme is capable of template-dependent nucleotide polymerization across discontinuous dsDNA templates. The products are shorter than those obtained on ssDNA templates. It prefers NTP over dNTP in template-dependent nucleotide polymerization. The enzyme synthesizes substantially more products on oligo(dC) with GTP as the substrate than on oligo(dT) with ATP as the substrate Saccharolobus solfataricus P2 N(pN)n + n diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
SsoPriSL
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 70 polymerization reactions on C35 or C34ddC with rGTP. Both the size and the quantity of the products on C35 increase, while synthesis on C34ddC drastically decreases, when the reaction temperature is raised from 55°C to 70°C Saccharolobus solfataricus