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Literature summary for 2.7.7.B16 extracted from

  • Augustin, M.A.; Huber, R.; Kaiser, J.T.
    Crystal structure of a DNA-dependent RNA polymerase (DNA primase) (2001), Nat. Struct. Biol., 8, 57-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion in sitting drops at 20°C. Crystal structure of the catalytic primase subunit, 2.3 A resolution Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the zinc ion is located within the loop region between beta-strand 6 and the prominent helix alpha4. It is coordinated by Cys 106 and His 108 of the loop and Cys114and Cys117 at the beginning of helix alpha4 Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q9P9H1 and Q8U4H7 Q9P9H1: small subunit, Q8U4H7: large subunit
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Purification (Commentary)

Purification (Comment) Organism
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Pyrococcus furiosus