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Literature summary for 2.7.7.9 extracted from
- Molecular cloning and analysis of the UDP-glucose pyrophosphorylase in Streptococcus equi subsp. zooepidemicus (2011), Mol. Biol. Rep., 38, 2751-2760.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant overepression in Streptococcus zooepidemicus, expression of His-tagged enzyme in Escherichia coli | Streptococcus equi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
UTP | pH 7.5, 37°C | Streptococcus equi |  |
| 0.0364 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C | Streptococcus equi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | Streptococcus equi | - |
diphosphate + UDP-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus equi | Q8GQP8 | subsp. zooepidemicus. i.e. Streptococcus zooepidemicus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme in Escherichia coli by nickel affinity chromatography | Streptococcus equi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Streptococcus equi | diphosphate + UDP-glucose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose pyrophosphorylase | - |
Streptococcus equi |
| UGPase | - |
Streptococcus equi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptococcus equi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.81 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C | Streptococcus equi | |
| 69.05 | - |
UTP | pH 7.5, 37°C | Streptococcus equi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptococcus equi |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structure-function analysis, homology modeling | Streptococcus equi |
| physiological function | the enzyme plays an important role in Streptococcus zooepidemicus cell envelope hyaluronic acid biosynthesis and it is also recognized as a virulence determinant in several bacterial species | Streptococcus equi |
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Release 2023.1 (January 2023)
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