Literature summary for 2.7.7.9 extracted from

Meng, M.; Fitzek, E.; Gajowniczek, A.; Wilczynska, M.; Kleczkowski, L.A.
Domain-specific determinants of catalysis/substrate binding and the oligomerization status of barley UDP-glucose pyrophosphorylase (2009), Biochim. Biophys. Acta, 1794, 1734-1742.

Cloned(Commentary)

Cloned (Comment)	Organism
His-tag version expressed in Escherichia coli BL21 (DE3)	Hordeum vulgare

Protein Variants

Protein Variants	Comment	Organism
Ccut-101	101 amino acid residues deleted (exons 16-19, and 1 amino acid residue of exon 15)	Hordeum vulgare
Ccut-32	32 amino acid residues deleted (exon 18 and exon 19)	Hordeum vulgare
Ccut-67	67 amino acid residues deleted (exons 17-19)	Hordeum vulgare
Ccut-8	8 amino acid residues deleted (last exon (exon 19))	Hordeum vulgare
Delta 1-4	deletion of 4 amino acid residues of the C-terminal domain, possibly involved in oligomerization	Hordeum vulgare
Delta 1-8	deletion of 8 amino acid residues of the C-terminal domain, possibly involved in oligomerization	Hordeum vulgare
Delta-NB	deletion mutant: amino acid residue 96-100 deleted (essential for catalysis)	Hordeum vulgare
K183A	possibly involved in subunit interaction	Hordeum vulgare
K260A	possibly involved in diphosphate binding	Hordeum vulgare
K332A	possibly involved in subunit interaction	Hordeum vulgare
K405A	possibly involved in subunit interaction	Hordeum vulgare
Ncut-21	21 amino acid residues deleted (exon 1)	Hordeum vulgare
Ncut-37	37 amino acid residues deleted (exon 1 and exon 2)	Hordeum vulgare
Y192A	possibly involved in UDP-glucose binding	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.027	-	diphosphate	Y192A mutant protein	Hordeum vulgare
0.03	-	diphosphate	Ccut-8 mutant protein	Hordeum vulgare
0.03	-	UDP-glucose	Ccut-8 mutant protein	Hordeum vulgare
0.03	-	diphosphate	K183A mutant protein	Hordeum vulgare
0.03	-	UDP-glucose	K183A mutant protein	Hordeum vulgare
0.03	-	UDP-glucose	K332A mutant protein	Hordeum vulgare
0.031	-	UDP-glucose	Y192A mutant protein	Hordeum vulgare
0.034	-	UDP-glucose	wild-type protein	Hordeum vulgare
0.039	-	diphosphate	wild-type protein	Hordeum vulgare
0.04	-	diphosphate	K332A mutant protein	Hordeum vulgare
0.04	-	UDP-glucose	K405A mutant protein	Hordeum vulgare
0.074	-	UDP-glucose	K260A mutant protein	Hordeum vulgare
0.09	-	diphosphate	Ncut-21 mutant protein	Hordeum vulgare
0.118	-	UDP-glucose	Ccut-101 mutant protein	Hordeum vulgare
0.24	-	diphosphate	K405A mutant protein	Hordeum vulgare
0.31	-	UDP-glucose	Ccut-32 mutant protein	Hordeum vulgare
0.362	-	UDP-glucose	Ccut-67 mutant protein	Hordeum vulgare
0.503	-	UDP-glucose	Delta 1-8 mutant protein	Hordeum vulgare
0.555	-	UDP-glucose	Delta 1-4 mutant protein	Hordeum vulgare
0.9	-	diphosphate	Ncut-37 mutant protein	Hordeum vulgare
1.1	-	UDP-glucose	Ncut-21 mutant protein	Hordeum vulgare
4.9	-	diphosphate	K260A mutant protein	Hordeum vulgare
6.4	-	diphosphate	Ccut-32 mutant protein	Hordeum vulgare
9.8	-	diphosphate	Ccut-67 mutant protein	Hordeum vulgare
29.4	-	diphosphate	Delta 1-4 mutant protein	Hordeum vulgare
33.3	-	diphosphate	Ccut-101 mutant protein	Hordeum vulgare
57	-	UDP-glucose	Ncut-37 mutant protein	Hordeum vulgare
60	-	diphosphate	Delta 1-8 mutant protein	Hordeum vulgare

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	Q43772	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography	Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
-	-	Delta-NB mutant protein	Hordeum vulgare
0.02	-	Ncut-37 mutant protein	Hordeum vulgare
0.21	-	Ccut-101 mutant protein	Hordeum vulgare
0.27	-	Ccut-32 mutant protein	Hordeum vulgare
0.42	-	K260A mutant protein	Hordeum vulgare
2.1	-	Delta 1-4 mutant protein	Hordeum vulgare
3.1	-	Ccut-67 mutant protein	Hordeum vulgare
5.4	-	Delta 1-8 mutant protein	Hordeum vulgare
12.8	-	Ncut-21 mutant protein	Hordeum vulgare
591	-	Y192A mutant protein	Hordeum vulgare
752	-	K183A mutant protein	Hordeum vulgare
764	-	K405A mutant protein	Hordeum vulgare
853	-	K332A mutant protein	Hordeum vulgare
1074	-	wild-type protein	Hordeum vulgare
1547	-	Ccut-8 mutant protein	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UTP + alpha-D-glucose 1-phosphate	-	Hordeum vulgare	diphosphate + UDP-glucose	-	?

Subunits

Subunits	Comment	Organism
oligomer	x * 52000-53000, SDS-PAGE	Hordeum vulgare

Synonyms

Synonyms	Comment	Organism
UDP-glucose pyrophosphorylase	-	Hordeum vulgare

pI Value

Organism	Comment	pI Value Maximum	pI Value
Hordeum vulgare	K183A mutant protein	-	5.47
Hordeum vulgare	K332A mutant protein	-	5.47
Hordeum vulgare	K405A mutant protein	-	5.47
Hordeum vulgare	Ncut-21 mutant protein	-	5.54
Hordeum vulgare	Delta 1-8 mutant protein	-	5.55
Hordeum vulgare	Delta-NB mutant protein	-	5.55
Hordeum vulgare	wild-type protein	-	5.55
Hordeum vulgare	Y192A mutant protein	-	5.55
Hordeum vulgare	Ccut-101 mutant protein	-	5.58
Hordeum vulgare	Delta 1-4 mutant protein	-	5.63
Hordeum vulgare	Ccut-67 mutant protein	-	5.69
Hordeum vulgare	Ncut-37 mutant protein	-	5.7
Hordeum vulgare	Ccut-32 mutant protein	-	5.79
Hordeum vulgare	Ccut-8 mutant protein	-	5.8