Cloned (Comment) | Organism |
---|---|
gene Ugp, expression in Escherichia coli | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
C99S | site-directed mutagenesis, half Vmax of wild-type, 12fold higher Km for diphosphate, altered diphosphate binding | Hordeum vulgare |
additional information | amino acid exchanges in hydrophobic domain | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
diphosphate | recombinant wild-type, pH 7.6, 25°C | Hordeum vulgare | |
0.065 | - |
UDP-glucose | recombinant wild-type, pH 7.6, 25°C | Hordeum vulgare | |
0.101 | - |
UDP-glucose | recombinant mutant C99S, pH 7.6, 25°C | Hordeum vulgare | |
0.407 | - |
diphosphate | recombinant mutant C99S, pH 7.6, 25°C | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
mixture of monomers, dimers and higher-order polymers, native PAGE and gel filtration, the monomer is the active form | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | Hordeum vulgare | key enzyme in biosynthesis of sucrose, cellulose, and other saccharides | diphosphate + UDP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q43772 | gene Ugp | - |
Purification (Comment) | Organism |
---|---|
native and recombinant wild-type, the latter from Escherichia coli to homogeneity | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare | diphosphate + UDP-glucose | - |
r | |
UTP + alpha-D-glucose 1-phosphate | key enzyme in biosynthesis of sucrose, cellulose, and other saccharides | Hordeum vulgare | diphosphate + UDP-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UGPase | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Hordeum vulgare |