Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT | Bos taurus |
General Stability | Organism |
---|---|
SH-reagents have no effect on enzyme stability | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-glucose 1-phosphate | product inhibition | Bos taurus | |
Mg-diphosphate | product inhibition | Bos taurus | |
MgUTP2- | - |
Bos taurus | |
additional information | no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT | Bos taurus | |
sucrose | 54% inhibition at 2% | Bos taurus | |
UTP | MgUTP2- | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus | |
Mg2+ | activation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
cow | - |
Purification (Comment) | Organism |
---|---|
533fold, multiple forms | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mammary gland | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
purified enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, crude acetone powder, desiccated storage, 2 years | Bos taurus |
PCMB, DTT, iodoacetate or 2-mercaptoethanol does not affect storage stability | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Bos taurus | diphosphate + UDP-glucose | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Bos taurus |
30 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, stable in 0.25 M potassium phosphate buffer, pH 7.5-9.0 | Bos taurus |
52 | - |
30 min, 70% loss of activity, 0.25 M potassium phosphate buffer, pH 7.5-9.0 | Bos taurus |
60 | - |
5 min, inactivation | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
4°C, 80% loss of activity immediately | Bos taurus |
4 | - |
4°C, t1/2: 2 days | Bos taurus |
5 | - |
86 days, 4°C, 70% loss of activity | Bos taurus |
6.5 | 7.5 | 86 days, 4°C, stable | Bos taurus |
7.5 | 9 | 30 min, stable at 50°C, in 0.25 M potassium phosphate buffer | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Bos taurus | |
0.135 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 30°C | Bos taurus | |
0.42 | - |
MgUTP2- | pH 8.0, 30°C | Bos taurus |