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Literature summary for 2.7.7.9 extracted from
- Purification, properties and kinetic analysis of UDP-glucose pyrophosphorylase from bovine mammary tissue (1972), Biochim. Biophys. Acta, 268, 102-112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| SH-reagents have no effect on enzyme stability | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-glucose 1-phosphate | product inhibition | Bos taurus |  |
| Mg-diphosphate | product inhibition | Bos taurus | |
| MgUTP2- | - |
Bos taurus | |
| additional information | no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT | Bos taurus | |
| sucrose | 54% inhibition at 2% | Bos taurus | |
| UTP | MgUTP2- | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | activation | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 500000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
cow | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 533fold, multiple forms | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mammary gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
purified enzyme | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude acetone powder, desiccated storage, 2 years | Bos taurus |
| PCMB, DTT, iodoacetate or 2-mercaptoethanol does not affect storage stability | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Bos taurus | diphosphate + UDP-glucose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Bos taurus |
| 30 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, stable in 0.25 M potassium phosphate buffer, pH 7.5-9.0 | Bos taurus |
| 52 | - |
30 min, 70% loss of activity, 0.25 M potassium phosphate buffer, pH 7.5-9.0 | Bos taurus |
| 60 | - |
5 min, inactivation | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
4°C, 80% loss of activity immediately | Bos taurus |
| 4 | - |
4°C, t1/2: 2 days | Bos taurus |
| 5 | - |
86 days, 4°C, 70% loss of activity | Bos taurus |
| 6.5 | 7.5 | 86 days, 4°C, stable | Bos taurus |
| 7.5 | 9 | 30 min, stable at 50°C, in 0.25 M potassium phosphate buffer | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Bos taurus | |
| 0.135 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 30°C | Bos taurus | |
| 0.42 | - |
MgUTP2- | pH 8.0, 30°C | Bos taurus | |
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Release 2023.1 (January 2023)
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