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Literature summary for 2.7.7.8 extracted from

  • Das, S.K.; Sokhi, U.K.; Bhutia, S.K.; Azab, B.; Su, Z.Z.; Sarkar, D.; Fisher, P.B.
    Human polynucleotide phosphorylase selectively and preferentially degrades microRNA-221 in human melanoma cells (2010), Proc. Natl. Acad. Sci. USA, 107, 11948-11953.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.8

Application

Application Comment Organism
medicine targeted overexpression of hPNPaseold-35 might provide an effective therapeutic strategy for miR-221-overexpressing and IFN-resistant tumors, such as melanoma Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information human melanoma cells are infected with empty adenovirus or with an adenovirus expressing hPNPaseold-35 and identification of miRNAs differentially and specifically regulated by hPNPaseold-35 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
melanoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
microR-221 RNAn+1 + phosphate recombinantly expressed microRNAs miR-let7a, miR-106b, miR-25, miR-221, miR-222, and miR-184 as substrates, the recombinant enzyme selectively and preferentially degrades microRNA-221 in human melanoma cells Homo sapiens microR-221 RNAn + ADP
-
 r
additional information human polynucleotide phosphorylase hPNPaseold-35 is a type I IFN-inducible 3'-5' exoribonuclease, which degrades specific mRNAs and small noncoding RNAs. miR-221, a regulator of the cyclin-dependent kinase inhibitor p27kip1, displays robust downregulation with ensuing up-regulation of p27kip1 by expression of hPNPaseold-35,which also occurs in multiple human melanoma cells upon IFN-beta treatment Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hPNPaseold-35
-
 Homo sapiens
polynucleotide phosphorylase
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction inhibition of the enzyme by shRNA or stable overexpression of miR-221 protects melanoma cells from IFN-beta-mediated growth inhibition Homo sapiens
additional information human melanoma cells infected with an adenovirus expressing hPNPaseold-35 and are used for identification of miRNAs differentially and specifically regulated by hPNPaseold-35. Overexpression of miR-221 in HO-1 cells confers resistance to IFN-beta-mediated growth arrest Homo sapiens
physiological function hPNPaseold-35 regulates the expression of specific miRNAs, importance of hPNPaseold-35 induction and miR-221 downregulation in mediating IFN-beta action, mechanism of miRNA regulation involving selective enzymatic degradation, overview Homo sapiens