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Literature summary for 2.7.7.8 extracted from

  • Brishammar, S.; Juntti, N.
    RNA-synthesizing enzymes in healthy and TMV-infected tobacco leaves. Partial purification and characterization to tobacco polynucleotide phosphorylase (1974), Arch. Biochem. Biophys., 164, 224-232.
    View publication on PubMed
Search for more literature for 2.7.7.8

Activating Compound

Activating Compound Comment Organism Structure
Bis-(3-aminopropyl)-amine optimal polymerization activity requires presence, 2fold increase in activity at 30 mM Nicotiana tabacum
dithiothreitol similar activation as with 2-mercaptoethanol Nicotiana tabacum
mercaptoethanol 20 mM, 3fold activation Nicotiana tabacum
NH4+ maximal activation at 100 mM Nicotiana tabacum
putrescine optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM Nicotiana tabacum
spermidine optimal polymerization activity requires polyamines, 1.4fold increase in activity at 30 mM Nicotiana tabacum
spermine optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM Nicotiana tabacum

General Stability

General Stability Organism
insensitive to freezing Nicotiana tabacum
stable against several freezing and thawing cycles Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
additional information polymerization reaction is not inhibited by diphosphate Nicotiana tabacum
phosphate 0.1 mM, complete inhibition of polymerization activity Nicotiana tabacum
Poly(A) inhibition of poly(U) synthesis Nicotiana tabacum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximal activation at 200 mm Nicotiana tabacum
Mg2+ Mg2+ or Mn2+ required for activity, maximal activation at 1 mM Mg2+, inhibition above Nicotiana tabacum
Mn2+ Mg2+ or Mn2+ required for activity, maximal activation at 0.06 mM Mn2+, inhibition above Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 sedimentation analysis Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 L. var Samsun, healthy and TMV-infected leaves
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephadex, enzyme from healthy and TMV-infected leaves Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
 incorporation of UDP into polynucleotide Nicotiana tabacum

Storage Stability

Storage Stability Organism
-75°C, several months, no loss of activity Nicotiana tabacum
4°C, 1 week, 50% loss of activity Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP
-
 Nicotiana tabacum poly(A)+1 + phosphate
-
 r
RNAn + a nucleoside diphosphate synthetic activity enhanced in presence of a primer Nicotiana tabacum RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of ADP Nicotiana tabacum RNAn+1 + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 60 min assay Nicotiana tabacum
35
-
 10 min assay Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 polymerization of ADP Nicotiana tabacum

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.8 approx. 40% of maximal activity at pH 7.6, approx. 70% of maximal activity at pH 8.8 Nicotiana tabacum