Literature summary for 2.7.7.8 extracted from

Nolden, W.T.; Richter, G.
polynucleotide phosphorylase from a cyanobacterium (Synechococcus sp.): subunit composition and properties (1982), Z. Naturforsch. C, 37c, 600-608.

No PubMed abstract available

Search for more literature for 2.7.7.8

Activating Compound

Activating Compound	Comment	Organism	Structure
KCl	maximal activation at 300 mM	Synechococcus sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Na2HPO4	0.0032 mM, complete inhibition	Synechococcus sp.
sucrose	50%, approx. 50% inhibition of partially purified enzyme	Synechococcus sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	-	Synechococcus sp.
Mg2+	preferentially activated by Mg2+	Synechococcus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	alpha4, 4 * 70000, SDS-PAGE	Synechococcus sp.
275000	-	native PAGE	Synechococcus sp.

Organism

Organism	UniProt	Comment	Textmining
Synechococcus sp.	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, DEAE-cellulose, gel filtration, poly(A)-Sepharose	Synechococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.2	-	-	Synechococcus sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RNAn + a nucleoside diphosphate	-	Synechococcus sp.	RNAn+1 + phosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	alpha4, 4 * 70000, SDS-PAGE	Synechococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Synechococcus sp.

pI Value

Organism	Comment	pI Value Maximum	pI Value
Synechococcus sp.	isoelectric focusing	-	4.2

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)