Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite | Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.6 | - |
- |
Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(G) + GDP | much lower activity than with ADP, activity depends on polyribonucleotide primer | Azotobacter vinelandii | poly(G)+1 + phosphate | - |
? | |
ribonucleoside 5'-diphosphate + phosphate | - |
Azotobacter vinelandii | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
RNAn + a nucleoside diphosphate | synthesis of poly(A): no primer addition required if large amounts of enzyme or Mg2+ are used, with small amounts of either component a primer is required, poly(G) synthesis: primer required | Azotobacter vinelandii | RNAn+1 + phosphate | - |
? |