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Literature summary for 2.7.7.8 extracted from
- Enzymatic synthesis of polynucleotides. IV. Purification and properties of polynucleotide phosphorylase of Azobacter vinelandii (1961), J. Biol. Chem., 236, 3303-3311.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter vinelandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite | Azotobacter vinelandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 39.6 | - |
- |
Azotobacter vinelandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(G) + GDP | much lower activity than with ADP, activity depends on polyribonucleotide primer | Azotobacter vinelandii | poly(G)+1 + phosphate | - |
? |  |
| ribonucleoside 5'-diphosphate + phosphate | - |
Azotobacter vinelandii | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
| RNAn + a nucleoside diphosphate | synthesis of poly(A): no primer addition required if large amounts of enzyme or Mg2+ are used, with small amounts of either component a primer is required, poly(G) synthesis: primer required | Azotobacter vinelandii | RNAn+1 + phosphate | - |
? | |
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