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Literature summary for 2.7.7.8 extracted from
- Polynucleotide phosphorylase (1972), The Enzymes, 3rd. Ed. (Boyer, P. D. , ed. ), 7, 533-574.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Poly(A) | inhibition of oligadenylate and oligouridylate phosphorolysis | Micrococcus luteus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | values for Km with polynucleotides longer than 20 nucleotides are much smaller than the Km for oligonucleotides which lies at approx. 0.05 mM | Escherichia coli | |
| 0.033 | - |
nonaadenylate | phosphorolysis reaction | Micrococcus luteus | |
| 0.05 | - |
heptauridylate | phosphorolysis reaction | Micrococcus luteus | |
| 0.25 | - |
tetrauridylate | phosphorolysis reaction | Micrococcus luteus | |
| 2.5 | - |
triadenylate | phosphorolysis reaction | Micrococcus luteus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | - |
Halobacterium salinarum | - |
- |
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | - |
Auxenochlorella pyrenoidosa | 9507 | - |
| nucleus | - |
Rattus norvegicus | 5634 | - |
| nucleus | HeLa cells | Homo sapiens | 5634 | - |
| ribosome | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | 5840 | - |
| soluble | - |
Escherichia coli | - |
- |
| soluble | - |
Pseudomonas aeruginosa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNAn + a nucleoside diphosphate | Spinacia oleracea | - |
RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | Escherichia coli | PNPase is involved in RNA degradation | RNAn+1 + phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris lumbricoides | - |
- |
- |
| Auxenochlorella pyrenoidosa | - |
- |
- |
| Cavia porcellus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Halobacterium salinarum | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Lactiplantibacillus plantarum | - |
- |
- |
| Micrococcus luteus | - |
- |
- |
| Neisseria meningitidis | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
| Triticum aestivum | - |
wheat | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Salmonella enterica subsp. enterica serovar Typhimurium | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Cavia porcellus | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Lactiplantibacillus plantarum | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Escherichia coli | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Homo sapiens | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Rattus norvegicus | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Spinacia oleracea | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Pseudomonas aeruginosa | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Halobacterium salinarum | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Neisseria meningitidis | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Auxenochlorella pyrenoidosa | |
| RNAn+1 + phosphate = RNAn + a nucleoside diphosphate | mechanism of elongation, overview | Ascaris lumbricoides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| epithelioma cell | - |
Rattus norvegicus | - |
| leaf | - |
Spinacia oleracea | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Rattus norvegicus | - |
| root | - |
Triticum aestivum | - |
| sperm | - |
Homo sapiens | - |
| urine | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNAn + a nucleoside diphosphate | - |
Spinacia oleracea | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Salmonella enterica subsp. enterica serovar Typhimurium | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Cavia porcellus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | specificity overview | Lactiplantibacillus plantarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Homo sapiens | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Spinacia oleracea | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Pseudomonas aeruginosa | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Halobacterium salinarum | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Neisseria meningitidis | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Micrococcus luteus | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Auxenochlorella pyrenoidosa | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | specificity overview | Ascaris lumbricoides | RNAn+1 + phosphate | - |
r | |
| RNAn + a nucleoside diphosphate | primer-dependent activity | Triticum aestivum | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | PNPase is involved in RNA degradation | Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn+1 + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Cavia porcellus | RNAn + a nucleoside diphosphate | - |
? | |
| RNAn+1 + phosphate | - |
Lactiplantibacillus plantarum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Escherichia coli | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Homo sapiens | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Rattus norvegicus | RNAn + a nucleoside diphosphate | - |
? | |
| RNAn+1 + phosphate | - |
Spinacia oleracea | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Pseudomonas aeruginosa | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Halobacterium salinarum | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Neisseria meningitidis | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Micrococcus luteus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Auxenochlorella pyrenoidosa | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | - |
Ascaris lumbricoides | RNAn + a nucleoside diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
synthesis of poly U | Micrococcus luteus |
| 45 | 55 | polymerization of UDP and CDP | Escherichia coli |
| 60 | - |
polymerization of ADP and GDP, phosphorolysis of poly A | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Micrococcus luteus |
| 55 | 60 | 10 min, no loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 8 | 9.5 | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
| 8 | 9.5 | - |
Cavia porcellus |
| 8 | 9.5 | - |
Lactiplantibacillus plantarum |
| 8 | 9.5 | - |
Escherichia coli |
| 8 | 9.5 | - |
Homo sapiens |
| 8 | 9.5 | - |
Rattus norvegicus |
| 8 | 9.5 | - |
Spinacia oleracea |
| 8 | 9.5 | - |
Pseudomonas aeruginosa |
| 8 | 9.5 | - |
Halobacterium salinarum |
| 8 | 9.5 | - |
Neisseria meningitidis |
| 8 | 9.5 | - |
Auxenochlorella pyrenoidosa |
| 8 | 9.5 | - |
Ascaris lumbricoides |
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Release 2023.1 (January 2023)
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