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Literature summary for 2.7.7.7 extracted from

  • Shimizu, M.; Gruz, P.; Kamiya, H.; Kim, S.R.; Pisani, F.M.; Masutani, C.; Kanke, Y.; Harashima, H.; Hanaoka, F.; Nohmi, T.
    Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases (2003), EMBO Rep., 4, 269-273.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
F12A no detectable incorporation of ribonucleotide triphosphate is observed with the wild-type enzyme, the mutant form F12A efficiently incorporates and extends ribonucleotide triphosphate, even in the absence of Mn2+ ions Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P2 the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P2 the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P1 the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P1 the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P1
-
-
-
Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exclusively incorporates 8-OH-GTP opposite adenine Saccharolobus solfataricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exclusively incorporates 8-OH-GTP opposite adenine Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus P1 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis. 2-OH-dATP is predominantly incorporated opposite guanine and thymine Saccharolobus solfataricus P1 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn DNA polymerase pol Y1 exclusively incorporates 8-OH-GTP opposite adenine. DNA polymerase pol Y1 incorporates 2-OH-dATP predominantly opposite guanine and thymine. DNA polymerase pol B1 incorporates 8-OH-GTP opposite adenine and cytosine. DNA polymerase pol B1 incorporates 2-OH-dATP opposite thymine Saccharolobus solfataricus P1 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exclusively incorporates 8-OH-GTP opposite adenine Saccharolobus solfataricus P1 diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA pol B1
-
Saccharolobus solfataricus
DNA pol Y1
-
Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the Y-family DNA polymerases promote mutagenesis through the erroneous incorporation of oxidized dNTPs during DNA synthesis Saccharolobus solfataricus