Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
E170A | complete loss of the 3'-5'exonuclease activity | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | at 100 mM KCl, 98%b loss of activity | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 4-10 mM Mg2+ | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, SDS-PAGE | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29753 | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 78 | at 25°C | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
t1/2: 7 min. Retains 20% of its polymerase activity after 1 h incubation in the absence of substrates | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.5 | - |
Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.8 | pH 6.5: about 65% of maximal activity, pH 8.8: about 50% of maximal activity | Archaeoglobus fulgidus |