Literature summary for 2.7.7.7 extracted from

Irimia, A.; Loukachevitch, L.V.; Eoff, R.L.; Guengerich, F.P.; Egli, M.
Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus (2010), Acta Crystallogr. Sect. F, 66, 1013-1018.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
determination of crystal structures of a binary Mg2+-form Dpo4DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4DNAdCTP/dGTP complexes with 8-oxoG in the template strand. Crystals are grown using the sitting-drop vapor-diffusion method by mixing equal amounts of Dpo4DNA complex solution and of a reservoir solution containing 1220% polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M magnesium acetate and 20 mMTris pH 7.5	Saccharolobus solfataricus

Crystallization (Comment)

Organism

determination of crystal structures of a binary Mg2+-form Dpo4DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4DNAdCTP/dGTP complexes with 8-oxoG in the template strand. Crystals are grown using the sitting-drop vapor-diffusion method by mixing equal amounts of Dpo4DNA complex solution and of a reservoir solution containing 1220% polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M magnesium acetate and 20 mMTris pH 7.5

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
DNA polymerase Dpo4	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)