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Literature summary for 2.7.7.7 extracted from

  • Khairnar, N.P.; Misra, H.S.
    DNA polymerase X from Deinococcus radiodurans implicated in bacterial tolerance to DNA damage is characterized as a short patch base excision repair polymerase (2009), Microbiology, 155, 3005-3014.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Deinococcus radiodurans

Protein Variants

Protein Variants Comment Organism
additional information a polX disruption mutant expressing 5'-deoxyribose phosphate lyase and a truncated polymerase domain is comparatively less sensitive to gamma-radiation at 10 kGy than a polX deletion mutant, both mutants show higher sensitivity to hydrogen peroxide Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
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-
-
Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
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-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme shows deoxynucleotide transferase activity, short patch DNA synthesis activity on heteropolymeric DNA substrate, 5'-deoxyribose phosphate lyase activity and base excision repair function in vitro Deinococcus radiodurans diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn the enzyme shows deoxynucleotide transferase activity, short patch DNA synthesis activity on heteropolymeric DNA substrate, 5'-deoxyribose phosphate lyase activity and base excision repair function in vitro Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase X
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 Deinococcus radiodurans
PolX
-
 Deinococcus radiodurans

General Information

General Information Comment Organism
physiological function involvement of deinococcal polymerase X in DNA-damage tolerance, possibly by contributing to DNA double-strand break repair and base excision repair Deinococcus radiodurans