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Literature summary for 2.7.7.7 extracted from

  • Frederick, A.M.; Davis, M.L.; Rice, K.P.
    Inhibition of human DNA polymerase beta activity by the anticancer prodrug cloretazine (2009), Biochem. Biophys. Res. Commun., 378, 419-423.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,3-bis[2-chloroethyl]-2-nitrosourea
-
Homo sapiens
cloretazine i.e. 1,2-bis[methylsulfonyl]-1-[2-chloroethyl]-2-[(methylamino)carbonyl]hydrazine Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Homo sapiens diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase beta
-
Homo sapiens
Klenow fragment
-
Homo sapiens
pol beta
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.074
-
Pol beta, at 25°C, pH 8.0 Homo sapiens cloretazine
0.092
-
Pol beta, at 25°C, pH 8.0 Homo sapiens 1,2-bis[methylsulfonyl]-1-[(methylamino)carbonyl]hydrazine
0.271
-
Pol beta, at 25°C, pH 8.0 Homo sapiens 1,3-bis[2-chloroethyl]-2-nitrosourea
0.307
-
Klenow fragment, at 25°C, pH 8.0 Homo sapiens 1,2-bis[methylsulfonyl]-1-[(methylamino)carbonyl]hydrazine
0.387
-
Klenow fragment, at 25°C, pH 8.0 Homo sapiens cloretazine
1.435
-
Klenow fragment, at 25°C, pH 8.0 Homo sapiens 1,3-bis[2-chloroethyl]-2-nitrosourea

General Information

General Information Comment Organism
physiological function Pol beta is a DNA polymerase specific to base excision repair that also possesses lyase activity capable of severing the phosphoester bond on the 3' carbon of the abasic ribose Homo sapiens