Literature summary for 2.7.7.7 extracted from

Takahashi, S.; Yonezawa, Y.; Kubota, A.; Ogawa, N.; Maeda, K.; Koshino, H.; Nakata, T.; Yoshida, H.; Mizushina, Y.
Pyranicin, a non-classical annonaceous acetogenin, is a potent inhibitor of DNA polymerase, topoisomerase and human cancer cell growth (2008), Int. J. Oncol., 32, 451-458.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens
expressed in Escherichia coli strain JMpbeta5	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism
10-epi pyragonicin	-	Bos taurus
10-epi-pyragonicin	-	Homo sapiens
10-epi-pyragonicin	-	Rattus norvegicus
19-epi jimenezin	-	Bos taurus
19-epi-jimenezin	-	Homo sapiens
19-epi-jimenezin	-	Rattus norvegicus
4-hydroxy-5-methyl-3-tetradecyl-dihydrofuran-2(3H)-one	-	Homo sapiens
jimenezin	-	Bos taurus
jimenezin	-	Homo sapiens
jimenezin	-	Rattus norvegicus
additional information	not inhibited by gamma-lactone	Bos taurus
additional information	isozyme DNA polymerase lambda is not inhibited by mucocin	Homo sapiens
additional information	not inhibited by gamma-lactone, mucocin, jimenezin, and 19-epi-jimenezin	Rattus norvegicus
mucocin	-	Bos taurus
mucocin	-	Rattus norvegicus
muconin	-	Bos taurus
muconin	-	Homo sapiens
muconin	-	Rattus norvegicus
pyragonicin	-	Bos taurus
pyragonicin	-	Homo sapiens
pyragonicin	-	Rattus norvegicus
pyranicin	potent inhibitor	Bos taurus
pyranicin	-	Brassica oleracea
pyranicin	potent inhibitor	Drosophila melanogaster
pyranicin	-	Escherichia coli
pyranicin	potent inhibitor	Homo sapiens
pyranicin	-	Oncorhynchus masou
pyranicin	potent inhibitor	Rattus norvegicus
pyranicin	-	Tequatrovirus T4
pyranicin	-	Thermus aquaticus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Brassica oleracea	-	-	-
Drosophila melanogaster	-	-	-
Escherichia coli	-	-	-
Homo sapiens	-	-	-
Oncorhynchus masou	-	cherry salmon	-
Rattus norvegicus	-	-	-
Tequatrovirus T4	-	-	-
Thermus aquaticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Drosophila melanogaster
-	Escherichia coli
-	Homo sapiens
-	Rattus norvegicus
-	Bos taurus
-	Brassica oleracea
-	Oncorhynchus masou

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Drosophila melanogaster	-
testis	-	Oncorhynchus masou	-
thymus	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
deoxynucleoside triphosphate + DNAn	-	Drosophila melanogaster	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Thermus aquaticus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Escherichia coli	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Homo sapiens	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Rattus norvegicus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Bos taurus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Tequatrovirus T4	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Brassica oleracea	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	-	Oncorhynchus masou	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
DNA polymerase	-	Drosophila melanogaster
DNA polymerase	-	Homo sapiens
DNA polymerase	-	Brassica oleracea
DNA polymerase alpha	-	Bos taurus
DNA polymerase beta	-	Rattus norvegicus
DNA polymerase delta	-	Oncorhynchus masou
DNA polymerase I	-	Thermus aquaticus
DNA polymerase I	-	Escherichia coli
DNA polymerase I	-	Tequatrovirus T4
Klenow fragment	-	Escherichia coli
pol alpha	-	Bos taurus
pol alpha	isozyme	Drosophila melanogaster
pol beta	-	Rattus norvegicus
pol delta	-	Oncorhynchus masou
pol delta	isozyme	Drosophila melanogaster
pol delta	isozyme	Homo sapiens
POl epsilon	isozyme	Drosophila melanogaster
POl epsilon	isozyme	Homo sapiens
Pol eta	isozyme	Homo sapiens
Pol gamma	isozyme	Homo sapiens
Pol I	-	Thermus aquaticus
Pol I	-	Escherichia coli
Pol I	-	Tequatrovirus T4
Pol I	isozyme	Brassica oleracea
Pol II	isozyme	Brassica oleracea
pol iota	isozyme	Homo sapiens
pol kappa	isozyme	Homo sapiens
Pol lambda	isozyme	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.0023	-	DNA polymerase lambda, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.0053	-	in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Bos taurus	pyranicin
0.0059	-	DNA polymerase gamma, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.0065	-	DNA polymerase alpha, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Drosophila melanogaster	pyranicin
0.0084	-	DNA polymerase delta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.0088	-	in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Oncorhynchus masou	pyranicin
0.0089	-	DNA polymerase delta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Drosophila melanogaster	pyranicin
0.0096	-	in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Rattus norvegicus	pyranicin
0.0102	-	DNA polymerase eta, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.0111	-	DNA polymerase kappa, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.013	-	DNA polymerase iota, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.014	-	DNA polymerase epsilon, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Drosophila melanogaster	pyranicin
0.0158	-	DNA polymerase epsilon, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Homo sapiens	pyranicin
0.2	-	IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Escherichia coli	pyranicin
0.2	-	IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Tequatrovirus T4	pyranicin
0.2	-	IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Brassica oleracea	pyranicin
0.2	-	IC50 above 0.2 mM, DNA polymerase I, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 74°C	Thermus aquaticus	pyranicin
0.2	-	IC50 above 0.2 mM, DNA polymerase II, in 50 mM Tris-HCl (pH 7.5), 1 mM dithiothreitol, 50% (v/v) glycerol, 0.1 mM EDTA, at 37°C	Brassica oleracea	pyranicin