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Literature summary for 2.7.7.7 extracted from

  • Wang, Y.; Musser, S.K.; Saleh, S.; Marnett, L.J.; Egli, M.; Stone, M.P.
    Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV (2008), Biochemistry, 47, 7322-7334.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of Dpo4 in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
ternary polymerase-DNA-dNTP, dGTP and/or dATP, complexes for three template-primer DNA sequences, 18-mer-primer 13-mer sequences containing 1,N2-propanodeoxyguanosine, with bound Ca2+, X-ray diffraction structure determination and analysis at 2.4-2.7 A resolution, modelling Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ bound to the active site, structure, overview Saccharolobus solfataricus
DTT
-
 Saccharolobus solfataricus
Mg2+
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus
-
 diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus P2
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W02
-
-
Saccharolobus solfataricus P2 Q97W02
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Dpo4 from Escherichia coli by heat treatment, nickel affinity and anion exchange chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn
-
 Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
 Saccharolobus solfataricus
Dpo4
-
 Saccharolobus solfataricus
More the enzyme belongs to the Y-family polymerases Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Saccharolobus solfataricus