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Literature summary for 2.7.7.7 extracted from

  • Sanders, L.H.; Rockel, A.; Lu, H.; Wozniak, D.J.; Sutton, M.D.
    Role of Pseudomonas aeruginosa dinB-encoded DNA polymerase IV in mutagenesis (2006), J. Bacteriol., 188, 8573-8585.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
gene dinB, transcription of the dinB gene is regulated as part of an SOS-like response, overexpression of C-terminally His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information deletion of the dinB gene sensitized Pseudomonas aeruginosa to nitrofurazone and 4-nitroquinoline-1-oxide, consistent with a role for DinB in translesion DNA synthesis over N2-dG adducts, while the UV-inducible mutator phenotype is independent of dinB function Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme is responsible for mutagenesis, e.g. UV-induced, in human host cells of lungs of cystic fibrosis patients contributing to morbidity and mortality of these people, with a striking correlation between mutagenesis and the persistence of Pseudomonas aeruginosa, mechanisms of mutagenesis, enzyme regulation, overview, the enzyme is responsible for resistance to to nitrofurazone and 4-nitroquinoline-1-oxide toxification ?
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Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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 gene dinB
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Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is responsible for mutagenesis, e.g. UV-induced, in human host cells of lungs of cystic fibrosis patients contributing to morbidity and mortality of these people, with a striking correlation between mutagenesis and the persistence of Pseudomonas aeruginosa, mechanisms of mutagenesis, enzyme regulation, overview, the enzyme is responsible for resistance to to nitrofurazone and 4-nitroquinoline-1-oxide toxification Pseudomonas aeruginosa ?
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Synonyms

Synonyms Comment Organism
DNA polymerase IV
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 Pseudomonas aeruginosa
More the enzyme belongs to the DNA polymerase Y family Pseudomonas aeruginosa