Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Frank, E.G.; Woodgate, R.
    Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese (2007), J. Biol. Chem., 282, 24689-24696.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ peak activity in the presence of Mg2+ is observed in the range of 0.1-0.5 mM and is significantly reduced at concentrations above 2 mM Homo sapiens
Mn2+ DNA polymerase iota exhibits the greatest activity in the presence of low levels of Mn2+ (0.05-0.25 mM). Mn2+ increases the catalytic activity of DNA polymerase iota by about 30000-60000fold through a strong decrease in the Km value for nucleotide incorporation. Whereas DNA polymerase iota preferentially misinserts G opposite T by a factor of about 1.4-2.5fold over the correct base A in the presence of 0.25 and 5 mM Mg2+, respectively, the correct insertion of A is actually favored 2fold over the misincorporation of guanine in the presence of 0.075 mM Mn2+. Low levels of Mn2+ also dramatically increase the ability of DNA polymerase iota to traverse a variety of DNA lesions in vitro. The cation utilized by DNA polymerase iota in vivo may be Mn2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Synonyms

Synonyms Comment Organism
DNA polymerase iota
-
Homo sapiens