Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlamydia pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia pneumoniae | - |
- |
- |
Chlamydia pneumoniae AR39 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Chlamydia pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | no detectable 3' exonuclease activity. CpDNApolI-dependent DNA synthesis is performed using DNA templates carrying different lesions. DNAs containing 2'-deoxyuridine (dU), 2'-deoxyinosine (dI) or 2'-deoxy-8-oxo-guanosine (8-oxo-dG) serve as templates as effectively as unmodified DNAs for CpDNApolI. Furthermore, the CpDNApolI can bypass natural apurinic/apyrimidinic sites (AP sites), deoxyribose (dR), and synthetic AP site tetrahydrofuran (THF). CpDNApolI can incorporate any dNMPs opposite both of deoxyribose and tetrahydrofuran with the preference to dAMP-residue. CpDNApolI preferentially extends primer with 3'-dAMP opposite deoxyribose during DNA synthesis, however all four primers with various 3'-end nucleosides (dA, dT, dC, and dG) opposite THF can be extended by CpDNApolI. Efficiently bypassing of AP sites by CpDNApolI is hypothetically attributed to lack of 3' exonuclease activity | Chlamydia pneumoniae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no detectable 3' exonuclease activity. CpDNApolI-dependent DNA synthesis is performed using DNA templates carrying different lesions. DNAs containing 2'-deoxyuridine (dU), 2'-deoxyinosine (dI) or 2'-deoxy-8-oxo-guanosine (8-oxo-dG) serve as templates as effectively as unmodified DNAs for CpDNApolI. Furthermore, the CpDNApolI can bypass natural apurinic/apyrimidinic sites (AP sites), deoxyribose (dR), and synthetic AP site tetrahydrofuran (THF). CpDNApolI can incorporate any dNMPs opposite both of deoxyribose and tetrahydrofuran with the preference to dAMP-residue. CpDNApolI preferentially extends primer with 3'-dAMP opposite deoxyribose during DNA synthesis, however all four primers with various 3'-end nucleosides (dA, dT, dC, and dG) opposite THF can be extended by CpDNApolI. Efficiently bypassing of AP sites by CpDNApolI is hypothetically attributed to lack of 3' exonuclease activity | Chlamydia pneumoniae AR39 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpDNApolI | - |
Chlamydia pneumoniae |
DNA polymerase I | - |
Chlamydia pneumoniae |
DNA-dependent DNA polymerase | - |
Chlamydia pneumoniae |