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Literature summary for 2.7.7.7 extracted from
- Cloning, expression, and characterization of DNA polymerase from hyperthermophilic bacterium Aquifex pyrophilus (2004), J. Microbiol. Biotechnol., 14, 1022-1030.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Aquifex pyrophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration is 20 mM | Aquifex pyrophilus |  |
| Mg2+ | optimal concentration is 3 mM | Aquifex pyrophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex pyrophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aquifex pyrophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | the enzyme contains a double strand-dependent 3'-5' proofreading exonuclease activity, but lacks any 5'-3' exonuclease activity | Aquifex pyrophilus | diphosphate + DNAn+1 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aquifex pyrophilus |
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