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Literature summary for 2.7.7.7 extracted from
- phi29 DNA polymerase requires the N-terminal domain to bind terminal protein and DNA primer substrates (1998), J. Mol. Biol., 278, 741-755.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| truncated enzyme | Salasvirus phi29 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Salasvirus phi29 |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
C-terminal fragment, SDS-PAGE | Salasvirus phi29 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | Salasvirus phi29 | - |
diphosphate + DNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salasvirus phi29 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Salasvirus phi29 | diphosphate + DNAn+1 | - |
? | |
| deoxynucleoside triphosphate + DNAn | 44kDa C-terminal fragment has no exonuclease activity, reduced efficiency with Mn2+ and reduced capacity to initiate terminal protein-primed DNA replication | Salasvirus phi29 | diphosphate + DNAn+1 | - |
? | |
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Release 2023.1 (January 2023)
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